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Simultaneous detection of IFN-gamma and IL-4 in lesional tissues and whole unstimulated saliva from patients with oral lichen planus |
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| Authors: | Xiao-an Tao Chun-yang Li Nelson L Rhodus Juan Xia Xiao-ping Yang Bin Cheng |
| Institution: | Department of Oral Medicine, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China;;Division of Oral Medicine, Oral Diagnosis &Radiology, Department of Diagnostic and Surgical Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, USA;;Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China. |
| Abstract: | Background: Although the roles of interferon (IFN)-gamma and interlekin (IL)-4 in oral lichen planus (OLP) have been described extensively in past decades, the available results are controversial. Moreover, few studies have utilized simultaneous detection of cytokines in local tissues and saliva to determine whether salivary cytokines could reflect the fact of local lesions. Methods: IFN-gamma and IL-4 were determined simultaneously in lesions and whole unstimulated saliva (WUS) from OLP patients with various clinical forms. Results: In OLP lesions, both IFN-gamma and IL-4 in erythematous/ulcerated OLP were higher significantly than that in control specimens. In WUS, however, only IFN-gamma of erythematous/ulcerated OLP was increased compared with control. Remarkably, IFN-gamma and IL-4 in WUS showed a more significant correlation to those in local tissues of all subjects. Conclusion: These results indicate that both IFN-gamma and IL-4 may play more important role in pathogenesis of erythematous/ulcerated OLP, and changes of these proinflammatory cytokines in WUS may reflect the status of the OLP lesion. |
| Keywords: | enzyme-linked immunosorbent assay interferon-gamma interleukin-4 oral lichen planus saliva |