| 红细胞抗原免疫印迹法在抗SSA抗体分型中的应用 |
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| 引用本文： | 刘学明 Mout.,HM.红细胞抗原免疫印迹法在抗SSA抗体分型中的应用[J].中国免疫学杂志,1994,10(1):41-43. |
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| 作者姓名： | 刘学明 Mout. HM |
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| 作者单位： | 白求恩医科大学第三临床医学院中心实验室 |
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| 摘 要： | 建立了以人红细胞为ＳＳＡ抗原来源的免疫印迹法（ＲＢＣ－ＩＢＴ）检测ＳＳＡ抗体。此法的敏感性略低于常规的对流免疫电泳法（ＣＩＥ），但可检出部分ＣＩＥ中阴性的ＳＳＡ抗体阳性血清，而且尚可鉴定出ＳＳＡ抗体的两种亚型（５２ＫＤ和６０ＫＤ）。检测了干燥综合症（ＳＳ）、系统性红斑狼疮（ＳＬＥ）及类风湿性关节炎（ＲＡ）等三组病人血清，其中ＳＳ和ＳＬＥ两组在ＳＳＡ抗体亚型的分布上差异显著。抗５２ＫＤＳＳＡ抗体主要分布于ＳＳ组（Ｐ＜０．０５），而抗６０ＫＤＳＳＡ抗体主要见于ＳＬＥ组（Ｐ＜０．０５），ＲＢＣ－ＩＢＴ中，非特异或不明显色带极少见，而ＳＳＡ抗体带清晰、易辨。
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| 关 键 词： | 免疫印迹 抗体 红细胞 抗原 |
DETERMINATION OF TH SUBTYPES OF ANTI-SSA ANTIBODIES BY IMMUNOBLOTTING
IN RBC EXTRACTS |
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| Liu Xueming,Moutsopoulos HM. Center. Lab.DETERMINATION OF TH SUBTYPES OF ANTI-SSA ANTIBODIES BY IMMUNOBLOTTING
IN RBC EXTRACTS[J].Chinese Journal of Immunology,1994,10(1):41-43. |
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| Authors: | Liu Xueming Moutsopoulos HM Center Lab |
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| Institution: | Liu Xueming,Moutsopoulos HM. Center. Lab.Third
Hospital Bethune University of Medical Sciences,Changchun 130021 |
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| Abstract: | or the detection of anti-SSA antibodies,immunoblotting using
human red blood cell ex-tracts(RBC-IBT) was established.The
sensitivity of RBC-IBT was slight lower than CIE,hoW-ever,it can
discover some of anti-SSA positive sera which were previously
negative in CIE. Fur-thermore,RBC-IBT can be able to recognize two
subtypes of anti-SSA antibodies(52KD and60KD).The prevalences of
these two subtypes were investigated in three groups of
patients(SS,SLE and RA),and significant differences between SS and
SLE groups have been found.Anti-52KD SSA were mainly distributed in
SS group (P<0.05),while anti-60KD SSA weremainly in SLE
group(P<0.05).Only a few non-specific bands can be seen in
RBC-IBT,andmake the specific bands of anti-SSA more clear and the
results more correct. |
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| Keywords: | Immunoblotting anti-SSA antibody RBC antigen |
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