| 卵巢上皮癌相关肿瘤抗原新基因的研究 |
| |
| 引用本文： | 王建东,章文华,吴令英,杨光,杨治华. 卵巢上皮癌相关肿瘤抗原新基因的研究[J]. 中国优生与遗传杂志, 2010, 0(1): 12-15 |
| |
| 作者姓名： | 王建东 章文华 吴令英 杨光 杨治华 |
| |
| 作者单位： | 首都医科大学附属北京妇产医院;中国医学科学院肿瘤医院; |
| |
| 摘 要： | 目的探讨卵巢癌相关肿瘤抗原新基因在卵巢癌诊断和免疫治疗中的应用价值。方法利用网上生物信息学分析软件对筛选到的55个克隆的核苷酸序列进行分析,并检测其中8个新基因在卵巢癌患者和正常女性血清中的抗体。结果55个克隆的核苷酸序列代表45个卵巢癌抗原基因,其中8个是国内外首次发现的在GeneBank中尚未登记的新基因,是具有免疫原性的功能基因。其中OCY-142新基因是抑癌基因BARD1的可剪切变异体,可被CD4+和CD8+T细胞识别。8个卵巢癌肿瘤抗原新基因中7个在卵巢癌患者血清中的阳性率显著高于正常人,7个卵巢癌肿瘤抗原新基因对卵巢癌患者和正常女性进行联合检测,其敏感性和特异性达到96.9%和95.2%。CAl25异常的卵巢癌41例患者中,OCY-142单独检测的阳性率为95.12%,而CA125正常的卵巢癌57例患者中,OCY-142检测发现21例(36.9%)阳性。结论这些卵巢癌相关肿瘤抗原新基因在卵巢癌患者血清中产生的相应抗体可能成为卵巢癌的新的血清学标志物,可能是卵巢癌免疫治疗理想的新靶点。
|
| 关 键 词： | 卵巢癌 肿瘤抗原 肿瘤相关基因 肿瘤标志物 免疫治疗 |
Study on ovarian cancer related novel antigen genes |
| |
| WANG Jian-dong,ZHANG Wen-hua,WU Ling-ying,YANG Guang,YANG Zhi-hua. Study on ovarian cancer related novel antigen genes[J]. Chinese Journal of Birth Health & Heredity, 2010, 0(1): 12-15 |
| |
| Authors: | WANG Jian-dong ZHANG Wen-hua WU Ling-ying YANG Guang YANG Zhi-hua |
| |
| Affiliation: | WANG Jian-dong,ZHANG Wen-hua1,WU Ling-ying1,YANG Guang1,YANG Zhi-hua1(Beijing Obstetrics , Gynecology Hospital,Capital Medical University 100026,1.Cancer Hospital,Peking Union Medical College) |
| |
| Abstract: | Objective:To evaluate the roles of novel antigen genes in diagnosis and immunothreapy of ovarian cancer.Methods:The genes selected by SEREX and SSH in our previously research works were identified after analysis by bioinformatics.8 novel antigen phage clones were screened using allogenic sera from 98 patients with ovarian serous papillary cystadenoma.Results:The sequencing results indicated that 55 positive clones represent 45 tumor antigen genes.8 of them are novel genes,and they all have immunogenicity.OC... |
| |
| Keywords: | Ovarian cancer Tumor antigen Tumor related gene Tumor marker Immunotherapy |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
