川芎嗪对兔失血性休克再灌注中脑的保护效应

目的:探讨川芎嗪对休克过程脑保护作用的效应及机制。方法:建立兔失血性休克模型,随机分为假手术组(对照组)、失血性休克组(休克组)、休克 回输血 等量NS组(灌注组)和休克 回输血 等量NS 川芎嗪(80 mg/kg)组(灌注 川芎嗪组)4组,每组8只。失血休克组于休克2 h时,灌注组、灌注 川芎嗪组在干预2 h时分别检测脑组织中琥珀酸脱氢酶(SDH)活性、血清和脑组织髓过氧化物酶(MPO)活性及丙二醛(MDA)含量,分别进行组间比较。结果:休克组、灌注组脑组织中SDH活性与对照组比较均降低,差异有统计学意义(P<0.01),与灌注 川芎嗪组比较明显升高,差异有统计学意义(P<0.01)。休克组、灌注组脑组织和血浆中MDA含量、MPO活性与对照组比较均升高,差异有统计学意义(P<0.01),与灌注 川芎嗪组比较明显降低,差异有统计学意义(P<0.01)。结论:川芎嗪对失血性休克代谢障碍及脑组织细胞具有显著的保护作用,可能与通过清除氧自由基、改善线粒体功能,减轻组织休克再灌注损伤有关。

Protective effects of salidroside on the brain of rabbits with hemorrhagic shock and reperfusion

Objective: To study the protective effects of Salidroside on brain metabolism in the hemorrhagic shock through establishing the rabbit model of hemorrhagic shock and reperfusion.Methods: Thirty two rabbits were randomly divided into four groups: the sham operation group(n=8),the rabbit model of hemorrhagic shock group(n=8),the rabbit model of hemorrhagic shock with intravenous injection of 0.9% saline group(n=8) and the rabbit model of hemorrhagic shock with intravenous injection of 0.9% saline and salidroside(80 mg/kg) group(n=8).2 hours after the sham operation,the hemorrhagic shock,the intravenous injection of 0.9% saline and the intravenous injection of 0.9% saline and salidroside(80 mg/kg) were given to each group respectively,the succinate dehydrogenase(SDH) activity in the brain tissue,the myeloperoxidase(MPO) activity and the malondialdehyde(MDA) content in the serum and brain tissue were measured carefully.Results: Compared with the sham operation group,SDH activity in the brain tissue of the hemorrhagic shock group decreased significantly(P<0.01),but in the salidroside group,the levels of the enzyme were higher than that in the hemorrhagic shock group and intravenous injection of 0.9% saline group(P<0.01).MDA and MPO in the brain tissue and serum of the hemorrhagic shock group and intravenous injection of 0.9% saline group were higher than those in hemorrhagic shock group(P<0.01),Both of them lowered greatly in the salidroside group(vs.the hemorrhagic shock group and intravenous injection of 0.9% saline group,(P<0.01).Conclusion: Salidroside may obviously protect the metabolism disorders and the injury of brain cells from hemorrhagic shock by scavenging oxygen free radicals,improving mitochondrial function,reducing organizations shock and reperfusion injury.