环氧化酶-2、血管内皮生长因子-C和CD44v6在胰腺癌中的表达及其与淋巴结转移的关系

目的 探讨胰腺癌组织中环氧化酶(COX)-2、血管内皮生长因子(VEGF)-C和CD44v6蛋白的表达及与胰腺癌病理学特征的关系.方法 采用免疫组织化学(ABC)法检测48例胰腺癌组织和14例癌旁组织中COX-2、VEGF-C和CD44v6蛋白的表达.结果 48例患者的胰腺组织中,COX-2、VEGF-C和CDd4v6均呈高表达,阳性表达率分别为79.2%、58.3%、62.5%,而在癌旁组织中均未见表达,差异有统计学意义(P<0.01).COX-2、VEGF-C和CD44v6蛋白表达与性别、分期、分化程度无明显相关(P均>0.05),而VEGF-C和CD44v6与淋巴结转移正相关(P<0.05).COX-2与VEGF-C蛋白表达相关(P<0.05),而与CD44v6无明显相关(P>0.05).结论 COX-2、VEGF-C、CD44v6蛋白在胰腺癌组织中呈高表达,VEGF-C、CD44v6表达与肿瘤的有无淋巴结转移有关,可作为胰腺癌浸润、转移的重要指标.

Expression of cyclooxygenase-2, vascular endothelial growth factor-c and CD44v6 proteins and its relation with lymphatic metastasis of pancreatic cancer

Objective To investigate the expression of cyclooxygenase-2 (COX-2), vascular en-dothelial growth factor-c (VEGF-C) , CD44v6 in pancreatic cancer tissue and its relation with the ctinico-pathologic features. Methods The expression of COX-2, VEGF-C and CD44v6 proteins in 48 cases of pancreatic cancer was detected by immnohistochemical staining. Results The positive rate of COX-2, VEGF-C and CD44v6 protein in pancreatic cancer tissue was 79.2% ,58.3%, and 62.5%, respectively, which was higher than in adjacent tissues (0/14) ( P < 0.01 ). The positive expression rate of COX-2, VEGF-C and CD44v6 was not correlated with sex, clinical stage and differentiation (P > 0.05 ), but that of VEGF-C and CD44V6 remarkably with lymph node metastasis ( P < 0.05 ). The expression of COX-2 was closely correlated with VEGF-C ( P < 0.05 ), but not with CD44v6 ( P > 0.05 ). Conclusion The expres-sion of COX-2, VEGF-C and CD44v6 in pancreatic cancer tissue might play a role in lymphatic metastasis via lymphangiogenesis.