Na~+-K~+-ATP酶活性与缺血半暗带脑组织缺血再灌注损伤的研究

目的探讨Na+-K+-ATP酶活性与缺血半暗带(IP)脑组织再灌注损伤的关系。方法 75只雄性Wistar大鼠随机分为假手术组15只,模型组60只。模型组又根据缺血再灌注时间分为6、24、48及72h4个时间点,每个时间点15只。模型组采用线栓法制备缺血2h再灌注模型。2组大鼠分别于再灌注6、24、48、72h断头取脑,观察IP脑组织Na+-K+-ATP酶活性、神经症状评分、脑组织水肿程度及脑梗死范围的变化。结果与假手术组比较,模型组大鼠IP脑组织Na+-K+-ATP酶活性明显降低(P<0.05),大鼠神经症状评分、脑组织含水量及脑梗死面积明显升高(P<0.05)。随着缺血再灌注时间的延长,大鼠神经症状评分、脑组织含水量均在48h升至最高,IP脑组织Na+-K+-ATP酶活性在48h降至最低,脑梗死面积在72h达最大值。结论在IP脑组织缺血再灌注损伤过程中,Na+-K+-ATP酶活性的改变起着至关重要的作用。

Relation between Na+-K+-ATPase activities and I/R injury in ischemic penumbra tissue

Objective To study the relation between Na+-K+-ATPase activities and I/R injury in ischemic penumbra tissue.Methods Seventy-five male Wistar rats were randomly divided into sham operation group(n=15) and model group(n=60).The model group was further divided into 6,24,48 and 72 h I/R groups(15 in each group).An I/R model was established with thread method.Brain tissue was taken out from the rats at 6,24,48 and 72 h after reperfusion.The Na+-K+-ATPase activities,neurological symptoms,brain edema and cerebral infarction size were observed.Results The Na+-K+-ATPase activities,neurological symptom scores,brain water content and cerebral infarction size were significantly higher in model group than in sham operation group(P<0.05).The neurological symptom scores and brain water content reached their peak at 48 h,the Na+-K+-ATPase activities reached their minimum at 48 h,the cerebral infarction size reached its peak at 72 h after I/R injury.Conclusion The Na+-K+-ATPase activities play a vital role in rat penumbra tissue I/R injury.