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| 套式PCR法检测间日疟原虫的研究 | |
| 引用本文： | 孙明林 陈培霞. 套式PCR法检测间日疟原虫的研究[J]. 地方病通报, 1996, 11(1): 17-20 |
| 作者姓名： | 孙明林 陈培霞 |
| 作者单位： | 第四军医大学寄生虫学教研室 |
| 摘 要： | 以滤纸采集间日疟原虫患者血样，煮沸法萃取ＤＮＡ模板，用间日疟原虫ＳＳＵｒＤＮＡ特异序列为内外引物，进行双温度点套式ＰＣＲ扩增，其检测原虫率水平为０．８４×１０＾－６，特异性强。检测云南疟区发热患者血样１６６份，检出率为６３．９％，显著高于镜检法的４８．２％。以镜检法为参照，本法的敏感性为９７％，特异性为６１％，阳性预期值和阴性预期值分别为６５％和９７％，表明是检测间日疟原虫感染的一个良好手段，可作 |
| 关 键 词： | 疟疾 间日疟原虫 聚合酶链反应 基因诊断 |
Study on Detection of Plasmodium uiuax in Blood Sample fromMalaria-Endemic Area by the Nested Polymerase Chain Reaction |
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| Sun Minglin,Chen Peixia,Xue Caifang,Wan Lei,Liu Zhongxiang. Study on Detection of Plasmodium uiuax in Blood Sample fromMalaria-Endemic Area by the Nested Polymerase Chain Reaction[J]. Endemic Diseases Bulletin, 1996, 11(1): 17-20 | |
| Authors: | Sun Minglin Chen Peixia Xue Caifang Wan Lei Liu Zhongxiang |
| Abstract: | |
| Keywords: | Plasmodium uiuax Small subunit ribosemal DNA Nested polymerase chain reaction Ge-netic diagnosis This project was supported by the Nationak Natural Science Foundation of China |
| 本文献已被 CNKI 维普 等数据库收录！ | |