Bcl-x 在不同子宫内膜组织中的表达及意义*

目的:探讨Bcl-xl 和Bcl-xs 在子宫内膜癌发生、发展中的作用，阐明二者的相关性。方法:采用RT-PCR 方法及Western-blot 法检测32例子宫内膜癌组织，12例非典型增生子宫内膜组织，6 例单纯性增生子宫内膜组织及10例正常子宫内膜组织中Bcl-xl 及Bcl-xs 的表达情况。结果:Bcl-xl mRNA 及蛋白在子宫内膜癌组织中表达显著高于正常子宫内膜组织（P 均<0.05），与子宫内膜癌病理分级有关（F=5.33，P=0.02）；Bcl-xs mRNA 及蛋白在非典型增生子宫内膜及子宫内膜癌组织表达与正常子宫内膜相比差异有显著性（P 均<0.05），与子宫内膜癌临床分期、淋巴结转移有关（P 均<0.05）；不同子宫内膜组织中Bcl-xl 与Bcl-xs 表达呈负相关（r=-0.76）。 结论:Bcl-xl 及Bcl-xs 的异常表达是子宫内膜癌发病的分子机制之一，二者在不同子宫内膜组织中的表达有相关性，二者之间的比例对子宫内膜癌的发生有一定影响。 

Expression of Bcl-x and Its Clinical Significance in Endometrial Cancer

Objective: To investigate the roles of Bcl-xl and Bcl-xs in the development and progression of endometrial carcinoma, and to explore their correlation.Methods: The expression of Bcl-xl and Bcl-xs in 32 cases of endometrial carci-noma, 12 cases of endometrial atypical hyperplasia, 6 cases of endometrial simple hyperplasia and 10 cases of normal en-dometrial tissues were examined by RT-PCR and Western-blot.Results: The expression of Bcl-xl mRNA and protein was significantly higher in endometrial cancer tissues than in normal endometrial tissues (P<0.05), and was statistically associat-ed with the pathological stage of endometrial carcinoma.(F=5.33, P=0.02).The expression of Bcl-xs mRNA and protein in atypical endometrial hyperplasia and endometrial carcinoma tissues were significantlly lower than that in normal endometri-al tissues (P<0.05), which was also associated with clinical stage and lymph node metastasis of endometrial carcinoma (P<0.05).The expression of Bcl-xl was negatively correlated with the expression of Bcl-xs in different endometrial tissues (r=-0.76).Conclusion: The abnormal expression of Bcl-xs and Bcl-xl was a factor for the pathogenesis and development of endometrial carcinoma.The negative correlation between Bcl-xl and Bcl-xs in different endometrial tissues as well as their relative expression ratio may have certain impact on the genesis of endornetrial cancer.