| 隐孢子虫免疫学检测方法研究进展 |
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| 引用本文： | 杨红玉,菅复春,张素梅,张龙现. 隐孢子虫免疫学检测方法研究进展[J]. 国际医学寄生虫病杂志, 2003, 36(1): 178-182. DOI: 10.3760/cma.j.issn.1673-4122.2009.03.012 |
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| 作者姓名： | 杨红玉 菅复春 张素梅 张龙现 |
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| 作者单位： | 河南农业大学牧医工程学院,郑州,450002; |
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| 基金项目： | 国家卫牛公益行业科研专项经费河南省重大公益性科研项目 |
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| 摘 要： | 隐孢子虫病是一种重要的人兽共患机会性致病原虫病,可通过食源和水源等途径传播,主要引起免疫抑制人群和易感动物腹泻,有重要的公共卫牛意义.免疫学检测方法有特异性强、灵敏度高等特点,应用广泛.该文主要介绍了免疫荧光榆测、酶免疫分析法、免疫层析法、酶联免疫电转印法、免疫磁珠分离法、流式细胞仪技术等几种常用的隐孢子虫免疫学检测方法,并初步分析了各种方法的优、缺点.
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| 关 键 词： | 隐孢子虫 免疫学检测方法 腹泻 |
Advance on immunologic detection of Cryptosporidium |
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| YANG Hong-yu,JIAN Fu-chun,ZHANG Su-mei,ZHANG Long-xian. Advance on immunologic detection of Cryptosporidium[J]. International JOurnal of Medical Parasitic Diseases, 2003, 36(1): 178-182. DOI: 10.3760/cma.j.issn.1673-4122.2009.03.012 |
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| Authors: | YANG Hong-yu JIAN Fu-chun ZHANG Su-mei ZHANG Long-xian |
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| Abstract: | Cryptosporidiasis is an important zoonosis caused by the opportunistic pathogenic protozoa Cryptosporidium transmitted through water and food.It may pose a significant public health risk by causing se-vere.or chronic diarrhoea in immunocompromised persons and susceptible animals.Immunologic detection meth-ods have been widely applied owing to its high specificity and sensitivity.Immunofluorescence assay(IFA),en-zyme immunoassay(EIA),immunochrumatography(IC),enzyme-linked immunoelectrotransfer blot(EITB),immunomagnetic beads separation(IMS)and flow cytometry(FC)in the immunologic detection of Cryptospo-ridium were reviewed,and the advantages and disadvantages of each method were briefly analyzed in this pa-per. |
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| Keywords: | CryptosporidiumImmunologic detection methodDiarrhoea |
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