桂红胶囊中银杏叶总黄酮醇苷的含量测定

目的:建立测定桂红胶囊中银杏叶总黄酮醇苷含量的方法。方法:用HPLC方法测定桂红胶囊中银杏叶总黄酮醇苷含量。以甲醇-0.4%磷酸(50:50)为流动相,检测波长为370 nm,流速为1.0 m L/min。结果:槲皮素在0.066~1.054μg范围内线性关系良好(r=0.9999,n=5),山柰素在0.068~1.628μg范围内线性关系良好(r=0.9999,n=5),异鼠李素在0.017~0.266μg范围内线性关系良好(r=0.9999,n=5);平均加样回收率:槲皮素为99.60%(RSD=2.53%,n=6),山柰素为99.00%(RSD=2.77%,n=6),异鼠李素为99.68%(RSD=2.61%,n=6)。结论:该法操作简便,灵敏度高、重现性好,可用于桂红胶囊中银杏叶总黄酮醇苷的含量测定。

Content Determination of Total Flavonoids Glycosides of Ginkgo Biloba in Guihong Capsule

Objective:To establish a method for content determination of total flavones glycosides of ginkgo biloba in Guihong capsule. Method :High performance liquid chromatography(HPLC) was applied to determine flavonoids glycosides. The mobile phase was methanol-phosphate acid(0.4%)(50:50),and the detective wavelength was 370 nm;the flow rate was 1.0 m L/min,Results:the linear ranges of quercetin,kaempferide and isorhamnetin were 0.066~0.527 μg,0.068~0.814 μg(r= 0.9999,n= 5) and 0.017~0.133 μg,respectively. The average recoveries of these three compounds were 99.60%(RSD= 2.53%,n= 6),99.00%(RSD= 2.77%,n= 6) and99.68%(RSD= 2.61%,n= 6),respectively. Conclusion:This method was simple,sensitive,repeatable,and could be used for the content determination of total flavonoids glycosides in the ginkgo biloba extract.