小鼠骨髓间充质干细胞在体内向肝细胞的转化

为探讨小鼠骨髓间充质干细胞(Bonemarrowmesenchymalstemcells,BMSCs)在体内向肝细胞转化的可能性,将来源于6-8周龄雄性BALB/c小鼠的BMSCs,通过尾静脉注射或经皮肝穿刺直接注入肝脏组织的途径移植入同源正常雌鼠体内。分别于移植术后1、2、4周处死受体雌鼠,取其肝组织,通过荧光原位杂交法(FISH)和免疫组化法同时检测Y染色体的存在和白蛋白的表达,以观察确定所注入的BMSCs在受鼠体内向肝细胞转化的情况。采取经皮直接肝穿刺注射途径移植BMSCs的两个实验组,无论移植细胞是新鲜分离的、还是P3代BMSCs,均可于移植后1周,在受鼠肝脏内检出Y染色体阳性细胞的存在,此类阳性细胞并可同时表达白蛋白。另一方面,经尾静脉注射法进行移植的实验组,于移植后2周,也可在其肝组织内检测到同时表达Y染色体和白蛋白的细胞。本研究证明了骨髓间充质干细胞在体内向肝细胞转化的可能性。

Diffentiation of Mouse Bone Marrow Mesenchymal Stem Cells Into Hepatocyte in vivo

To explore the possibility of mouse bone marrow mesenchymal stem cells(BMSCs) differentiating into hepatocyte in vivo, the BMSCs isolated from donor male BALB/C mice at age of 6-8 weeks were transplanted to female BALB/C mice as recipients by transfusion via tail vein or direct liver injection. By the end of 1 st, 2 nd, 4 th weeks after transplantation, the Y chromosome positive and albumin expression positive hepatocytes were detected from the recipient female mouse liver tissue by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). In the two groups of direct liver injection, no matter whether the donor cells are fresh isolated bone marrow cells or passage 3 cells, the Y chromosome positive cells with albumin expression simultaneously were detected by the end of the 1 st week after transplantation. Similar result has been observed in the other subgroup of tail vein injection, but only by the end of the 2 nd week after transplantation. BMSCs are capable of differentiating into hepatocyte in vivo.