温病湿热证大鼠IL-17的变化及加味藿朴夏苓汤干预对其的影响

目的：观察温病湿热证大鼠血清、舌组织白介素17（IL-17）表达水平的变化,以及加味藿朴夏苓汤干预对其的影响,探讨IL-17在温病湿热证发病中的作用和意义.方法：96只大鼠随机分为正常组、模型组、干预组.多因素复合造模方法制备温病湿热证大鼠模型,干预组第25天起予加味藿朴夏苓汤治疗,连服7d.于造模后4,24,48,72h4个时点分批处死大鼠并取材.ELISA法检测大鼠血清IL-17的水平,SP免疫组化法检测舌组织IL-17的表达.结果：模型组大鼠血清和舌组织IL-17的表达水平在各时点均高于正常组（P ＜0.01）,随时点变化均呈逐渐下降趋势,在4h时最高;随病情加重模型组大鼠血清和舌组织中IL-17的表达水平均呈逐渐升高趋势,均与病情的严重程度呈正相关（血清：r =0.671,P＜0.01;舌组织：r=0.714,P＜0.01）.干预组大鼠血清和舌组织IL-17的表达水平随时点变化呈下降趋势,在各时点均低于模型组（P＜0.01）而高于正常组（P＜0.05,P＜0.01）;随病情加重干预组大鼠血清和舌组织IL-17的表达水平均呈逐渐升高趋势,血清中重度组与轻度、中度组比较差异均有统计学意义（P ＜0.01,P＜0.05）;在舌组织中各组间比较差异亦有统计学意义（P＜0.01,P＜0.05）,与病情的严重程度仍呈正相关（血清：r =0.476,P＜0.01;舌组织：r =0.546,P＜0.01）.结论：IL-17参与了温病湿热证的发生、发展过程,其表达水平与温病湿热证的病情严重程度密切相关.加味藿朴夏苓汤可降低温病湿热证大鼠IL-17的表达,抑制炎症反应而减轻病情严重程度.

THE CHANGE OF IL-17 IN RAT WITH SYNDROME OF DAMP-HEAT AND THE INTERVENTION EFFECT OF MODIFIED HUOPUXIALING DECOCTION

Objective：To observe the changes of levels of IL-17 in serum and tongue tissues and the intervention effect of modified huopuxialing decoction in order to study the effect and significance of IL-17 in the pathogenesis of damp-heat syndrome.Methods：First,96 rats were randomly divided into normal group,damp-heat model group and medicine interference group.The damp-heat syndrome rat model was established by method of combining multi-factor.Medicine interference group was intervened by the modified huopuxialing decoction from the first 25 days.Dosage was divided into two gavage and taken continuously for 7 days.At 4 h,24 h,48 h,72 h after intervention,rats were sacrificed and sampled.The level of IL-17 in rat serum was detected by ELISA.The expression of IL-17 in rat tongue tissues was detected by SP-immunohistochemistry.Results：The levels of expression of IL-17 in serum and tongue tissues of model group was significantly higher than those in the normal group in each time point（P ＜0.01）.The levels of IL-17 was the highest in model 4h,and decreased gradually with time point.The serum and tongue mucosa contents of IL-17 showed a gradual increasing trend with the clinical symptoms,and was positively correlated to the severity of syndrome of damp-heat significantly（serum：r =0.671,P ＜0.01.tongue mucosa：r =0.714,P ＜0.01）.The levels of IL-17 in serum and tongue mucosa of medicine interference group were significantly lighter than the model group in each time point（P ＜0.01）but higher than the normal group （P ＜0.01,P ＜ 0.05）.The medicine interference group of IL-17 showed a gradual increasing trend with the clinical symptoms.Severe rat model group of IL-17 was significant differences in comparison with the mild,moderate group （P ＜0.01,P ＜0.05）.The tongue mucosa of IL-17 expression levels at each time point were relatively have different（P ＜0.01,P ＜0.05）.The remaining indicators are positively correlated with the severity of the disease （serum：r =0.476,P ＜0.01.tongue