Polypeptide synthesized in herpes simplex virus type 2-infected HEp-2 cells.

The proteins induced by herpes simplex virus type 2 in infected HEp-2 cells were studied by high-resolution polyacrylamide slab-gel electrophoresis. A total of 51 infected cell-specific polypeptides (ICSP) were detected, accounting for three-fourths of the coding capacity of the virus. The induced polypeptides were allocated to three groups based on their time of synthesis in the virus growth cycle. Cycloheximide treatment of infected cells during the early stage of virus growth was found to cause irreversible inhibition of protein synthesis. Herpes simplex virus type 2 polypeptides which underwent considerable posttranslational modification were detected.