哮喘患儿CD4+CD25+T细胞FoxP3表达的意义

目的研究表达FoxP3的CD4^＋ CD25^＋调节性T细胞在哮喘儿童外周血中的比例改变,探讨其在发病机制中的意义。方法采用细胞内染色的流式细胞术及定量PCR方法,分别在蛋白质和mRNA水平检测哮喘患儿外周血FoxP3表达,并与健康对照组进行比较。结果哮喘患儿CD4和CD25双阳性细胞所占比例与健康对照组比较无明显差异,而CD4^＋ CD25^high和CD4^＋ CD25^＋ FoxP3＋细胞明显低于对照组（Pa〈0.05）。FoxP3 mRNA表达水平与蛋白质表达水平变化一致。结论哮喘患儿CD4^＋ CD25^＋ FoxP3＋调节性T细胞明显减少,可能与哮喘的发病机制有关。

Significance of Expression of Intracellular FoxP3 in CD4+CD25+T Cells in Children with Asthma

Objective To investigate the potential role of FoxP3-expressing CD4 CD25 regulatory T cells in children with asthma.Met-hods Intracellular 3-colour flow cytometry and real time PCR were used to assess the expression of FoxP3 in peripheral blood mononuclear cell(PBMC)of asthma,repectively.Results There were similar frequencies of CD4 CD25 T cells in peripheral blood of asthma patients and healthy subjects.However,the percentage of CD4 CD25high and CD4 CD25 FoxP3 in asthma group were lower than those in healthy subjects(Pa<0.05).The result of real time PCR was consistent with that of flow cytometry.Conclusion The proportion of CD4 CD25 FoxP3 regulatory T cells decreased in children asthma,which may contribute to the pathological process of such disease.