|
|||||
|
|
Type-I interferon receptor expression: Its circadian rhythm and downregulation after interferon-α administration in peripheral blood cells from renal cancer patients |
|
| Authors: | Masahiro Shiba Norio Nonomura Yasutomo Nakai Masashi Nakayama Hitoshi Takayama Hitoshi Inoue Akira Tsujimura Kazuo Nishimura Akihiko Okuyama |
| Affiliation: | Department of Urology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan |
| Abstract: | Objectives: To investigate the regulation of interferon-α (IFN-α) receptor expression in metastatic renal cell carcinoma (RCC) after IFN-α administration. Methods: Blood sampling was carried out in eight patients with metastatic RCC and six healthy volunteers. Flow-cytometric analysis using a monoclonal antibody against the active subunit of the type-I IFN-α receptor (IFNAR2) was carried out to examine the circadian rhythm of IFNAR2 expression in peripheral blood mononuclear cells (PBMC) as well as its downregulation after IFN-α administration. Results: According to its circadian rhythm IFNAR2 in PBMC had a peak expression at night. Once IFN-α is administered, IFNAR2 levels in PBMC showed downregulation within 48 h and recovered within another 48 h. Conclusions: Our findings might support the establishment of an optimal schedule for IFN-α administration. |
| Keywords: | circadian rhythm downregulation interferon receptor renal cell carcinoma |