输精管阻断对大鼠生精细胞增殖和生精过程的影响

为了解输精管阻断对大鼠生精细胞增殖和生精过程的影响，应用流式细胞技术，对输精管阻断４周和８ 周组大鼠的生精细胞ＤＮＡ 含量进行了分析。结果显示：输精管阻断４ 周和８ 周，实验组的精子细胞和精子比例明显少于相应的对照组（Ｐ＜ ０．０５）；而精原细胞和次级精母细胞比例则明显高于相应的对照组（Ｐ＜ ０．０５）；同时，输精管阻断后生精细胞增殖指数（ＰＩ）值明显降低（Ｐ＜ ０．０５），且细胞多处于Ｇ０ ／Ｇ１ 期，其中８ 周组的Ｓ期细胞比例明显地少于４ 周组（Ｐ＜ ０．０５）。提示：输精管阻断可以影响生精过程的多个阶段，使生精细胞的增殖延缓。

The Effect of Vasectomy on Spermatogenesis and Proliferation of Rat Spermatogenic Cell

In order to explore the effect of vasectomy on spermatogenesis and proliferation of rat spermatogenic cell, we detected the DNA content of rat spermatogenic cells by flow cytometry at 4 weeks and 8 weeks after vasectomy. The results showed that the percentages of 1C cell group (spermatid and sperm) in the two experimental groups were apparently lower than that of the two control groups respectively ( P<0.05) , but the percentage of 2C cell group (spermatogonium and spermotocyte) in the two experiment groups were much higher than those of the two control groups respectively ( P <0 05); The proliferation index (PI) of experimental groups was significantly lower than that of control groups ( P <0 05) and most of the cells in the experimental groups were on G 0/G phase. The percentage of S phase cell in the 8 weeks experimental group was obviously lower than that of the 4 weeks experimental group. These findings suggest that vasectomy in rat may affect many stages in spermatogenesis and delay the proliferation of spermatogenic cell.