A rapid molecular diagnosis of posttransfusion graft-versus-host disease by polymerase chain reaction |
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Authors: | S Hayakawa ; F Chishima ; H Sakata ; K Fujii ; K Ohtani ; K Kurashina ; J Hayakawa ; K Suzuki ; H Nakabayashi ; M Esumi ; |
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Institution: | Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan. |
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Abstract: | A woman with recurrent Paget's disease of the vulva developed acute graft-versus-host disease (GVHD) 12 days after radical surgery and massive blood transfusion. Molecular diagnosis of lymphocyte chimerism in the peripheral blood was made by polymerase chain reaction (PCR) directed against a Y chromosome-specific sex-determining region Y (SRY) gene. PCR with skin biopsy after onset of GVHD also revealed infiltration of SRY-positive donor lymphocytes. The diagnosis was confirmed by HLA-DNA typing with PCR-sequence-specific oligonucleotide that revealed the presence of complex HLA-DR chimerism in the peripheral lymphocytes collected after onset of GVHD. The use of SRY- directed PCR is a rapid technique for the early diagnosis of acute posttransfusion GVHD in female patients. |
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