NO及细胞因子与急性胰腺炎预后的相关性研究

目的探讨血清NO及IL-8、IL-10、IL-18水平与急性胰腺炎预后的相关性。方法采用硝酸还原酶法及ELISA法分别测定正常对照组、轻型急性胰腺炎（mild acute pancreatitis,MAP）组与重症急性胰腺炎（severe acute pancreatitis,SAP）组第1、4、7、14天的NO及IL-8、IL-10、IL-18水平。结果急性胰腺炎组的血清NO、IL-8、IL-10及IL-18高于正常对照组（P〈0.05）,重症急性胰腺炎组NO、IL-8、IL-10及IL-18明显高于正常对照组（P〈0.01）。重症急性胰腺炎组NO、IL-8及IL-18高于急性胰腺炎组（P〈0.05）,但IL-10低于急性胰腺炎组（P〈0.05）。结论 NO及IL-8、IL-10及IL-18与急性胰腺炎严重程度密切相关,其血清水平可作为判断急性胰腺炎的严重程度指标之一。

Study on the relationship between serum levels of NO,IL-8,IL-10 and IL-18 and the prognosis of patients with acute pancreatitis

Objective To study the relationship between serum levels of NO,IL-8,IL-10 and IL-18 and the prognosis of patients with acute pancreatitis.Methods Serum levels of NO,IL-8,IL-10 and IL-18 were measured by colorimetric assay and ELISA methods in all patients with mild acute pancreatitis（MAP） or severe acute pancreatitis（SAP） on 1st,4th,7th,and 14th days compared with those from 30 healthy subjects.Results The serum levels of NO,IL-8,IL-10 and IL-18 in MAP as well as in SAP patients were higher than those of healthy subjects,Furthermore,the serum levels of NO,IL-8,IL-18 of patients with SAP were significantly higher than those of patients with MAP,but IL-10 was lower.Conclusion Cytokines IL-8,IL-10,IL-18 and NO were all markedly elevated in patients with acute pancreatitis.IL-8,IL-18 and NO were all positively correlated with the severity of acute pancreatitis.