纺锤体毒剂和断裂剂体外诱发V79细胞多核与微核效应的差异

采用长春新碱，秋水仙素，丝裂霉素Ｃ和环磷酰２胺对Ｖ７９细胞染毒，发现两类诱变剂在诱发多核与微核的敏感性和强度上存在显著差异。当剂量较高时，四种诱变剂都可诱发多核与微核，但纺锤体毒剂以请发多核为主，多核细胞率与微核细胞率的比值为２．０－３．５．而断裂剂以诱发微核为主。

DIFFERENTIAL EFFECTS OF ANEUGENS AND CLASTOGENS ON INCIDENCES OF MULTINUCLEATED CELLS AND OF MICRONUCLEATED CELLS IN CHINESE HAMSTER LUNG (V79) CELL LINE IN VITRO

Two aneugens: vinblastine (Vin) and colchicine(Col),and two clastogens: mitomycin C (MMC) and cyclophosphamide (CP), were applied to Chinese hamster lung(V79) cells respectively in vitro for micronucleus test. The results showed that the increases of incidence of multinucleated cells (Imu) and that of micronucleated cells (Imi ) in response to aneugens and clastogens were in different sensitivities. At relatively low concentrations, Vin and Col induced only Imu (but not Imi), while MMC induced only Imi; At higher concentrations, both Imu and Imi increased in a concentration dependent manner, however, Vin and Col induced multinuclei predominantly with the ratios of Imu to Imi 2.0-3.5,while MMC and CP induced micronuclei mainly, with Imu/Imi of 0.08-0.28. This intensity of difference in Imu/Imi was much greater than that in areas of micronuclei between aneugens and clastogens. The results indicate that in micronucleus test in vitro, observation of both Imu and Imi might be valuable for distinguishing aneugens from clastogens.