Local and systemic immune responses in humans againstHelicobacter pyloriantigens from homologous and heterologous strains |
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| Institution: | 1. Department of Medical Microbiology and Immunology, Göteborg University, Sweden;2. Department of Surgery, Göteborg University, Sweden;1. Clinical Hematology Unit, Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;2. Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;3. Tropical Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;4. Chest Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;5. Family Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;6. Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;7. Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;8. Gastroenterology and Hepatology Unit, Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt;9. Department of Materials, Imperial College London, London, SW7 2AZ, United Kingdom;10. Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia |
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| Abstract: | The capacity ofHelicobacter pylorito induce strain specific immune responses was studied in adult Swedish volunteers. Sera and gastric aspirates from 11H. pylori-infected subjects were tested for specific antibody levels against, respectively, lipopolysaccharides (LPS) and total membrane preparations (MPs) prepared from the study subjects» own strains, as well as with corresponding antigens from two referenceH. pyloristrains or heterologous strains collected from other subjects within the study. It was found that sera from five of the 11 subjects had significantly higher IgA antibody titres against LPS from the homologous strain than against LPS from either of the reference strains and in five cases sera reacted with higher IgG titres against the homologous LPS than with LPS preparations from the reference or heterologous patient strains. Analyses of specific titres against MPs revealed that six sera had higher IgA titres and four sera had higher IgG titres against MPs prepared from the subjects» own strains than against MPs from either of the two reference strains. Determination of specific antibodies in gastric aspirates revealed significantly higher IgA titres against LPS from the homologousH. pyloriisolate than against LPS from the two reference strains in five cases, and six aspirates reacted in higher IgA titre with the homologousH. pyloriMPs. Results from immunoblotting analyses of sera support induction of strain specific immune responses againstH. pyloriLPS. By means of specific monoclonal antibodies againstH. pyloriLPS, antigenic heterogeneity between the different LPS preparations tested was confirmed. |
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