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Cell mediated immunity induced in mice by HPV 16 L1 virus-like particles
Affiliation:1. Department of Biomaterials, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, College of Materials, Xiamen, PR China;2. State Key Laboratory of Physical Chemistry of Solid Surface, School of Chemistry and Chemical Engineering, Xiamen University, Xiamen, PR China;3. State Key Laboratory of Molecular Vaccinology and Molecular Diagnosis & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, PR China;4. Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen, PR China;5. Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, PR China;6. Chinese Center for Disease Control & Prevention Institute Viral Disease Control & Prevention, Beijing, PR China;1. Department of Medical Laboratory Sciences, Masinde Muliro University of Science and Technology, Kakamega, Kenya;2. Kenya Medical Research Institute, Nairobi, Kenya;3. Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya;4. Division of Adolescent and Young Adult Medicine, Department of Pediatrics, University of California, San Francisco, CA, USA
Abstract:Recombinant human papillomavirus (HPV) type 16 L1 virus-like particles (VLPs) expressed in the baculovirus system were used to investigate the cellular immune response to human papillomavirus type 16. The cell-mediated immune response was evaluated through immunization of mice with HPV 16 L1 virus-like particles using a lymphoproliferation assay and cytokine production and cytometric analysis of lymphocyte subsets. A significant proliferative response was observed which was associated with secretion of both interferon-γ and interleukin-2. FACS analysis of splenic lymphocytes revealed that CD8+T-cells were increased in the immunized mice. These results demonstrate that HPV 16 L1 VLPs induce a T-cell response characterized by a Th1 profile and confirm that the HPV 16 VLP is a reasonable candidate for vaccine development.
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