Involvement of inflammatory cytokines and nitric oxide in the expression of non-specific resistance toListeria monocytogenesin mice induced by viable but not killedMycobacterium bovisBCG |
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| Institution: | 1. Graduate Program in Animal Science, Universidade do Estado de Santa Catarina, Chapecó, Santa Catarina, Brazil;2. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil;3. Laboratory of Pathology, Instituto Federal Catarinense, Concórdia, Santa Catarina, Brazil;4. Graduate Program in Toxicological Biochemistry, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil;5. Pharmacy Laboratory, Faculdade Anhanguera, Pelotas, RS, Brazil;6. Laboratory of Biochemistry Research and Molecular Biology of Microorganisms (LaPeBBiOM), Universidade Federal de Pelotas, RS, Brazil;1. Department of Neurosurgery, Medical University of Vienna, Vienna, Austria;2. Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria;3. Institute of Neurology, Medical University of Vienna, Vienna, Austria;4. Department of Radiotherapy, Medical University of Vienna, Vienna, Austria;1. Division of Neurological Sciences, Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland;2. Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern CH-3012, Switzerland;3. Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, University of Bern, Bern CH-3001, Switzerland;4. Institute of Genetics, Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland;5. Department of Anatomy, University of Veterinary Medicine Hannover, Hannover D-30173, Germany;6. Veterinary Public Health Institute, Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Bern CH-3097, Switzerland;1. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico;2. Unidad Periférica del Instituto de Investigaciones Biomédicas en el Instituto Nacional de Neurología y Neurocirugía, Mexico;3. Dept of Immunology & Immunogenetics, Instituto de Diagnóstico y Referencia Epidemiológicos-InDRE, Secretaria de Salud, Ciudad de México, Mexico;4. Laboratorio de Diagnóstico Genómico, Instituto Nacional de Medicina Genómica, Ciudad de México, Mexico;5. Institut National de la Santé et de la Recherche Médicale (INSERM), UMR906, GIMP, Labex ParaFrap, Aix-Marseille Université, 13005 Marseille, France;1. Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia;2. Centro Nacional de Secuenciación Genómica—CNSG, Sede de Investigación Universitaria—SIU, Universidad de Antioquia, Colombia;3. Grupo Sistemas Embebidos e Inteligencia Computacional—SISTEMIC, Departamento de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Antioquia, Colombia;4. Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia—GICIG, Colombia |
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| Abstract: | The non-specific defense againstListeria monocytogenescould be induced by viable BCG but not by killed BCG in mice. In order to understand the mechanism of antilisterial activity, viable and killed BCG were compared for their ability of inducing cytokine gene expression in spleen cells. Both viable and killed BCG induced the same level of mRNA expression of interleukin 10 (IL-10), transforming growth factor beta (TGF-β), IL-12 and tumor necrosis factor alpha (TNF-α). Gene expression and production of IL-1αand gamma interferon (IFN-γ) could be induced by stimulation only with viable BCG. Viable BCG but not killed BCG induced the mRNA expression of inducible nitric oxide synthase (iNOS). Treatment of mice withNG-monomethyl-L-arginine acetate (NMMA) significantly impaired the non-specific antilisterial action induced by viable BCG. These results demonstrated that NO is an important mediator for the non-specific antilisterial activity induced by viable BCG, and IFN-γ, IL-1αand TNF-αmay play a critical role in the non-specific antilisterial activity. |
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