| HPLC法同时测定眼内灌注液中醋酸钠与枸橼酸的含量 |
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| 作者姓名： | 李永贵 冷佳蔚 张锡霞 王晶 |
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| 作者单位： | 1. 山东省药学科学院，山东省化学药物重点实验室，山东 济南 250101; 山东福瑞达医药集团公司，山东省黏膜与皮肤给药技术重点实验室，山东 济南 250101;2. 山东省食品药品检验研究院,山东 济南,250101 |
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| 摘 要： | 目的：建立高效液相色谱法同时测定眼内灌注液中醋酸钠与枸橼酸钠含量。方法采用 Cosmosil 5C18－PAQ 色谱柱（4．6 mm ×250 mm），磷酸水溶液（水用磷酸调节 pH 值至2．15）－甲醇（95∶5）为流动相，检测波长为210 nm，柱温30℃，流速1．0 mL·min －1。结果醋酸钠浓度在0．1411～0．3292 mg·mL －1范围内线性关系良好，平均回收率为100．71％，RSD 为0．57％；枸橼酸钠浓度在0．0895～0．2089 mg·mL －1范围内线性关系良好，平均回收率为100．63％，RSD 为0．54％。结论该方法简便、快速、准确、可靠、专属性强。
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| 关 键 词： | 高效液相色谱 眼内灌注液 醋酸钠 枸橼酸钠 含量测定 |
Simultaneous determination of sodium acetate and sodium citrate in Intraocular Perfusion Liquid by HPLC |
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| Authors: | LI Yonggui LENG Jiawei ZHANG Xixia and WANG Jing |
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| Institution: | 1.Shandong Academy of Pharmaceutical Sciences,Shandong Provincial Key Laboratory of Chemical Drug,Jinan 250101,China;2.Shandong Freda Pharmaceutical Group Company,Shandong Provincial Key Laboratory of Mucosal and Transdermal Drug Delivery Technology,Jinan 250101,China,3.Shandong Institute for Food and drug Control,Jinan 250101,China,1.Shandong Academy of Pharmaceutical Sciences,Shandong Provincial Key Laboratory of Chemical Drug,Jinan 250101,China;2.Shandong Freda Pharmaceutical Group Company,Shandong Provincial Key Laboratory of Mucosal and Transdermal Drug Delivery Technology,Jinan 250101,China and 1.Shandong Academy of Pharmaceutical Sciences,Shandong Provincial Key Laboratory of Chemical Drug,Jinan 250101,China;2.Shandong Freda Pharmaceutical Group Company,Shandong Provincial Key Laboratory of Mucosal and Transdermal Drug Delivery Technology,Jinan 250101,China |
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| Abstract: | Objective To establish an HPLC method for the simultaneous determination of sodium acetate and sodium citrate in Intraocular Perfusion Liquid.Methods This method was adopted with Cosmosil 5C1 8 -PAQ(4.6 mm ×250 mm) column,the mobile phase was consisted of hosphoric acid aqueous solution (water with phosphoric acid to adjust the pH to 2.1 5) -methanol (95∶5).The detection wavelength was 21 0 nm,the column temperature was 30 ℃,the flow rate was 1 .0 mL·min -1 .Results The linear range of sodium acetate was 0.1 41 1 ~0.329 2 mg·mL -1 ,the average recover was 1 00.71 % and the RSD was 0.57%;The linear range of sodium citrate was 0.089 5 ~0.208 9 mg·mL -1 ,the average re-cover was 1 00.63% and the RSD was 0.54%.Conclusion The method was simple,rapid,accurate,reliable and specific. |
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| Keywords: | HPLC Intraocular Perfusion Liquid Sodium acetate Sodium citrate Determination |
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