HPLC法测定咽喉清口含片中哈巴俄苷及肉桂酸的含量

目的：建立同时测定咽喉清口含片中哈巴俄苷和肉桂酸含量的高效液相色谱方法。方法采用Waters Symmetry C18（4.6 mm×250 mm，5μm）为固定相，以乙腈-0.3%醋酸水溶液为流动相进行梯度洗脱，检测波长为279 nm，流速为1.0 mL·min-1，柱温为30℃。结果哈巴俄苷进样量在0.03965~0.3965μg时，与峰面积呈良好线性关系（ r＝0.9998），平均回收率98.13%（ n＝6），RSD为1.20%；肉桂酸进样量在0.09968~0.9968μg范围内，线性关系良好（ r＝0.9997），平均回收率为98.87%（ n＝6），RSD为1.17%。结论所建立的方法准确可靠、重复性好，可用于咽喉清口含片中相关成分的测定，以控制该制剂质量。

Determination of harpagoside and cinnamic acid in Yanhouqing Buccal Tablets by HPLC

Objective To establish a method for the determination of harpagoside and cinnamic acid in Yanhouqing Buccal Tablets.Methods Separations were carried out on a Waters Symmetry C18 column(4.6 mm×250 mm,5μm)by gra-dient elution with 0. 3% acetic acid and acetonitrile,the detection wavelength was 279 nm,the flow rate was 1. 0 mL·min-1 ,and the column temperature was 30 ℃. Results Chromatographic peaks of harpagoside and cinnamic acid were well separated with good precision and stability.Then the standard curve of harpagoside was rectilinear within the range of 0.039 65~0.396 5 μg(r=0.999 8),and the average recovery was 98.13 % with RSD of 1.20 %(n=6).The standard curve of cinnamic acid was rectilinear within the range of 0.099 68~0.996 8μg( r=0.999 7),and the average recovery was 98.87 % with RSD of 1.17%(n=6).Conclusion The method was accurate and reproducible,and can be used for the de-termination of cinnamic acid and harpagoside in Yanhouqing Buccal Tablets.