黄皮茎枝的化学成分研究

目的：研究黄皮属植物黄皮茎枝 Clausena lansium （Lour．）Skeels 的化学成分。方法采用大孔吸附树脂、MCI 吸附树脂、硅胶柱色谱、反相柱色谱、凝胶柱色谱以及制备高效液相色谱等方法进行分离纯化，运用紫外光谱、质谱和核磁共振谱等方法鉴定化合物的结构。结果从黄皮茎枝的95％乙醇提取物的水萃取部位分离得到10个化合物，分别鉴定为β－腺苷（1）、伞形花内酯－7－O －α－L －鼠李糖基－（1″－6′）－β－D －葡萄糖苷（2）、Haploperoside A （3）、伞形花内酯－7－O －β－D －芹糖基－（1″－6′）－β－D －葡萄糖苷（4）、Marmesinin（5）、苯甲基－O －β－D －芹糖基－（1″－6′）－β－D －葡萄糖苷（6）、苯甲基－O －β－D －吡喃葡萄糖苷（7）、对羟基苯丙酸（8）、β－hydroxypropiovanillone （9），反式对羟基桂皮酸（10）。结论化合物1～6、8～9为首次从该属植物中分离得到。化合物1对脂多糖诱导的 BV2细胞产生一氧化氮具有一定的抑制活性，其 IC50为4．39μmoL·L －1。

Chemical constituents from the stems of Clausena lansium

Objective To study the chemical constituents from the stems of Clausena lansium (Lour.)Skeels.Meth-ods The chemical constituents were separated and purified by macroporous resin,MCI gel,silica gel,ODS column chroma-tography,Sephadex LH -20,and preparative HPLC,and their structures were determined by the analysis of ultraviolet spec-trum,mass spectrum,and nuclear magnetic resonance spectroscopy.Results Ten compounds were isolated from the H2 O fractions of 95% ethanol extract of the stems of Clausena lansium,and their structures were identified as β-Adenosine(1), Umbelliferone -7 -α-L -rhamnopyranosyl -(1″-6′)-β-D -glucopyranoside(2),Haploperoside A(3),Umbellifer-one -7 -O -β-D -rhamnopyranosyl -(1″-6′)-β-D -glucopyranoside(4),marmesinin(5),Benzyl -O -β-D -apiofuranosyl -(1″-6′)-β-D -glucopyranoside(6),Benzyl -O -β-D -glucopyranoside(7),3 -(4 -hydroxyphe-nyl)-propanoic acid(8),β-hydroxypropiovanillone (9),trans -p -hydroxycinnamic acid(10).Conclusion Com-pounds 1 ~6,8 ~9 were isolated from the plants of genus Clausena L.Compounds 1 showed some inhibitory effects on LPS-induced NO production in murine microglial BV2 cells (IC50 =4.39 μmoL·L -1 ).