rh-bFGF对缺血再灌注损伤兔视网膜组织中谷氨酸表达的影响

目的 探讨重组人碱性成纤维细胞生长因子(rh-bFGF)对缺血再灌注损伤(IRI)兔视网膜组织中谷氨酸水平的影响。 方法 将66只大耳白兔分为A组［视网膜缺血再灌注损伤(RIRI)组］30只、B组(RIRI+rh-bFGF组)30只和C组(正常假手术组)6只;缺血再灌注后6、12、24、48、72 h,A、B 2组又依次分为5个亚组,每组6只。造模初期,A、B组分别于玻璃体腔内注入相同剂量的生理盐水和rh-bFGF溶液,并于制模后5个时间点处死,摘取眼球。C组给予前房穿刺,无其他操作,处死后摘除眼球。观察3组视网膜病理形态学的变化,并检测视网膜谷氨酸含量。 结果 A组视网膜出现高度水肿,层次紊乱,细胞结构疏松,视网膜节细胞(RGC)出现空泡样变性,神经节细胞数目减少;B组视网膜结构层次和细胞组织变化与A组大致相同,但其损害程度较A组明显减轻;C组视网膜各层次清晰,细胞平行排列且形态规则,RGC呈单层排列、规则,无空泡变性。缺血再灌注后6、12、24、48、72 h,A、B 2组视网膜谷氨酸含量均显著高于C组(P<0.01)。与A组比较,B组各时间点谷氨酸含量明显降低(P<0.01)。 结论 rh-bFGF对兔视网膜损伤具有保护作用,其机制可能与抑制细胞凋亡、降低谷氨酸含量有关。

Effect of rh-bFGF on the level of glutamic acid in retinal tissue of rabbits with ischemia-reperfusion injury

Objective To explore the effect of recombinant human basic fibroblast growth factor (rh-bFGF)on the lev-el of glutamic acid in retinal tissue of rabbits with ischemia-reperfusion injury.Methods Sixty-six white rabbits were divided into group A[retinal ischemia-reperfusion injury (RIRI)group,n =30],group B (RIRI +rh-bFGF,n =30) and group C (sham-operation group,n =6).Groups A and B were also assigned into 5 subgroups respectively 6,12, 24,48 and 72 h after ischemia-reperfusion injury,6 rabbits in each group.The same dose of normal saline and rh-bFGF solution were respectively injected into the vitreous cavity of rabbits in groups A and B at the early stage of molding. The rabbits were killed and their eyeballs were taken out at five time points after molding.Puncture of anterior chamber was given in group C,and no other operations were given.The rabbits in group C were killed and their eyeballs were taken out.The changes of retinal pathomorphism were observed,and the contents of glutamic acid were detected. Results In group A,the retina was characterized by high edema,disordered levels and loose cell structure.Vacuole degeneration was present in retinal ganglion cells (RGC)and and the number of ganglion cells decreased.In group B, the retinal structure changes were similar to that in A group,but the damage was significantly milder than that in group A.In group C,the levels of retina were clear,the cells were arranged in parallel and retinal morphology was reguLar. RGC was reguLarly arranged in a single layer,and no vacuole degeneration occurred.6,12,24,48 and 72 h after ischemia-reperfusion injury,the contents of glutamic acid in groups A and B were dramatically higher than that in group C (P <0.01).When compared with group A,the contents of glutamic acid in group B decreased notably at different time points (P<0.01).Conclusion rh-bFGF has a protective effect on retinal injury in rabbits,and its mechanism may be related to the inhibition of cell apoptosis and decreased content of glutamic acid.