松杉灵芝发酵产物对H22肝腹水瘤小鼠免疫功能影响及抗肿瘤作用

目的 研究松杉灵芝发酵产物对H22肝腹水瘤小鼠免疫系统的影响及抗肿瘤活性.方法 采用H22肝腹水瘤小鼠模型,设生理盐水空白组、阳性药对照组、发酵产物高、中、低剂量组,每天灌胃给药1次,连续15d,末次给药后检测脾脏T淋巴细胞和B淋巴细胞增值情况,用酶联免疫分析法检测血清中免疫球蛋白(IgG)和白细胞介素2(IL-2)的含量,MTT法检测H22肿瘤细胞的增殖情况.结果 与空白对照组相比,松杉灵芝发酵产物各组的白细胞介素2、胸腺指数、抑瘤率、B细胞反应均显著提高,茵丝中、低剂量组T细胞增殖显著增加,茵丝低剂量组、发酵液高剂量组和中剂量组免疫球蛋白均显著提高.结论 松杉灵芝发酵产物可显著提高小鼠的免疫功能,有效抑制H22肝腹水瘤细胞的增殖.

The immune function and anti-tumor effects of Ganoderma tsugae fermentation product on H22 liver ascites tumor mice

Objective To study the influence of Ganoderma tsugae fermentation product on the immunological function of H22 liver ascites tumor mice and its anti-tumor effect.Methods H22 liver ascites tumor mice model was adopted,set physiological saline control group,positive control group,fermentation product high,medium and low dose group,intragastrically administered once a day,successively for 15 days.After the last administration, spleen T lymphocyte and B lymphocyte proliferation status were tested,and used ELISA to detect serum IgG and IL-2 index;MTT was used to detect killing effect on H22 tumor cells.Results Compared with the control group,Ganoderma tsugae fermentation product respective group had apparently increasing effect on IL-2,thymus index,anti-tumor rate and B cell reaction,mycelia medium and low dose group had obviously capacity to induce T cell proliferation,mycelia low dose group and fermentation high dose group and medium dose group had obviously increasing effect on IgG.Conclusion Ganoderma tsugae fermentation product could effectively increase mice immunological function,effectively restrain H22 liver ascites tumor proliferation,expanding mice life span.