血小板衍生生长因子-BB对血管内皮细胞、平滑肌细胞及成纤维细胞胶原蛋白合成的影响

目的:观察血小板衍生生长因子-BB对培养的人血管内皮细胞、平滑肌细胞和成纤维细胞胶原蛋白合成的影响.方法:采用培养的人血管内皮细胞、平滑肌细胞和成纤维细胞,应用3H-脯氨酸掺入方法,观察血小板衍生生长因子-BB对3种细胞胶原蛋白合成的影响.结果:血小板衍生生长因子-BB可促进处于静止状态的成纤维细胞和平滑肌细胞胶原蛋白合成,并呈现出明显的浓度依赖关系,成纤维细胞和平滑肌细胞胶原蛋白合成分别在30 ng/ml和40 ng/ml时达到高峰,血小板衍生生长因子-BB对血管内皮细胞胶原蛋白合成总量无明显促进作用.在30 ng/ml的血小板衍生生长因子-BB作用下,成纤维细胞和平滑肌细胞分别在36 h和48 h时胶原蛋白合成达到高峰.结论:血小板衍生生长因子-BB可明显促进培养的人血管平滑肌细胞和成纤维细胞的胶原蛋白的合成,对血管内皮细胞胶原蛋白合成总量无明显促进作用.

Effects of platelet-derived growth factor BB on collagen synthesis in human vascular endothelial cells, smooth muscle cells and fibroblasts

Objective:To investigate the effects of platelet-derived growth factor BB (PDGF-BB)on collagen synthesis in human vascular endothelial cells, smooth muscle cells and fibroblasts. Methods: The human vascular endothelial cells, smooth muscle cells and fibroblasts were cultured and the effects of PDGF-BB on collagen synthesis in the 3 kinds cells were observed by using 3H-proline incorporation in vitro. Results: PDGF-BB significantly promoted collagen synthesis in quiescent human smooth muscle cells and fibroblasts in a dose-dependent manner, with the peak synthesis in fibroblasts at 30 ng/ml and in smooth muscle cells at 40 ng/ml;when PDGF-BB was at 30 ng/ml,the peak synthesis in smooth muscle cells and fibroblasts were at 36 h and 48 h,respectively. PDGF-BB showed no effect on collagen synthesis in human endothelial cells. Conclusion: PDGF-BB can promote collagen synthesis in cultured human smooth muscle cells and fibroblasts, but has no effect on collagen synthesis in human endothelial cells.