Three-Generation Reproduction Study with Dioctyl Sodium Sulfosuccinate in Rats

Three-Generation Reproduction Study with Dioctyl Sodium Sulfosuccinatein Rats. MACKENZIE, K., HENWOOD, S., FOSTER, G., AKIN, F., DAViS,R., DEBAECKE, P., SISSON, G., AND MCKINNEY, G. (1990). Fundam.Appl Toxicol. 15, 53–62. Groups of 30 male and 30 femalerats (F0) were fed diets containing 0, 0. 1, 0.5, or 1.0% dioctylsodium sulfosuccinate (DSS) for 10 and 2 weeks, respectively.The F0 animals were then mated to produce an F1 litter. Groupsof 30 male and 30 female F1 animals were fed the same dose levelsfor at least 10 weeks postweaning, and the breeding programwas repeated to produce F2 animals. F3 animals were producedfrom F2 animals by the same procedure. The study was terminatedwith the F3 wean lings. Test diets were fed continuously throughoutthe study. All F0, F1, and F2 adults and F3 weanlings (one/sex/litter)were necropsied and given a macroscopic examination. There wereno effects on reproductive function for parental animals ofeither sex during any of the three generations in this study.At the highest dose level (1.0% DSS), body weights were lowerthan those of controls dunng the premating phase for males inall three generations and for F1 and F2 females. Body weightsfor F1 and F2 males and females in the 0.5% dose group werealso low during the premating phase. Pup weights on LactationDay 0 were significantly lower than those of controls only forthe high-dose group during the third generation. However, lowerpup weight gains in the mid-and high-dose groups resulted insignificantly lower pup weights on Day 21 for all three generations.Perinatal pup survival across three generations ranged from96 to 100% for the control and treated groups. Pup survivalranged from 95 to 100% for controls, from 98 to 100% for low-and mid-dose groups, and from 91 to 99% for the high-dose group.There were no treatment-related mortality and antemortem ormacroscopic observations. In summary, DSS administered in thediet to three successive generations of rats at levels of 0.5and 1.0% caused a reduction in body weights for parental malesin all generations and for F1 and F2 females, Pup weights atthe 0.5 and 1.0% dose levels were also lower than those of thecontrol in all three generations. However, the reduced bodyweights did not interfere with development of normal reproductiveperformance. DSS at levels up to 1.0% had no effects on thereproductive function of either sex in any generation and producedno treatment-related antemortem or macroscopic observations.