Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones |
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Authors: | Zhang Ting Li Jun-ling Xin Jian Ma Xiao-chao Tu Zeng-hong |
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Institution: | StateKeyLaboratory,ofDrugResearch,ShanghaiInstituteofMateriaMedica,ShanghaiInstituteforBiologicalSciences,ChineseAcademyofSciences,Shanghai201203,China |
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Abstract: | AIM: To compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones (FQ). METHODS: Lomefloxacin (LFLX), sparfloxacin (SPFX), ciprofloxacin (CPFX), and levofloxacin (LELX) were tested by comet assay and photodynamic DNA strand breaking activity under the different conditions of UVA irradiation. RESULTS: In comet assay, photogenotoxicity was evident at SPFX 1 mg/L, LFLX 5 mg/L, and CPFX 5 mg/L, and LELX 10 mg/L. In photodynamic DNA strand-breaking activity, SPFX and LFLX induced the conversion of the supercoiled form into the nicked relaxed form at 10-50 micromol/L, while CPFX at 25 micromol/L and LELX at 50 micromol/L. CONCLUSION: There were good correlations between the two methods to detect DNA damage induced by phototoxicity of fluoroquinolones. Photodynamic DNA strand breaking activity was a good method to detect DNA damage induced by photogenotoxicity of fluoroquinolones as well as comet assay. |
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Keywords: | fluoroquinolones phototoxic dermatitis comet assay |
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