早产儿视网膜病变易感性与血管内皮生长因子-A+405和-A936基因多态性的相关性

目的 探讨早产儿视网膜病变(ROP)易感性与血管内皮生长因子(VEGF)-A+ 405和VEGF-A936基因多态性的相关性。方法 99例ROP患儿（ROP组）和80例非ROP早产儿（对照组）纳入本研究。两组受检者的出生年龄、出生体重及给氧时间之间差异均无统计学意义(P＞0.05)。取得所有受检者监护人的知情同意后，抽取外周静脉血2 ml。采用焦磷酸测序法检测VEGF-A+ 405和VEGF-A936基因多态性表型、等位基因和基因频率。分析ROP与VEGF-A+ 405和VEGF-A936基因多态性的相关性。结果 VEGF-A+ 405存在CC、GG、CG 3种基因型，VEGF-A936存在CC、CT 2种基因型。ROP组VEGF-A+405位点C、G等位基因分别为92、106，基因频率分别为46.5％，53.5％；对照组VEGF-A+405位点C、G等位基因分别为90、70，基因频率分别为56.2％，43.8％；两组比较，差异无统计学意义(X2=3.396，P=0.066)。相关性分析发现，VEGF-A+ 405基因多态性与ROP发生无相关性(OR=0.675，OR95％ CI=0.444，1.026)。ROP组VEGF-A936位点T、C等位基因分别为32、166，基因频率分别为16.2％，83.8％；对照组VEGF-A936位点T、C等位基因分别为16、144，基因频率分别为1o.o％，90.0％；两组比较，差异无统计学意义(X2 =2.894，P=0.089)。相关性分析发现，VEGF-A936基因多态性与ROP发生无相关性(OR=0.768，OR95％ CI=0.711，0.829)。结论 VEGF-A+ 405和VEGF-A936基因多态性与ROP易感性无关。

The correlation between retinopathy of prematurity susceptibility and +405G/C and 936 T/C polymorphism of vascular endothelial growth factor-A gene

ObjectiveTo investigate the correlation between retinopathy of prematurity(ROP)susceptibility and +405G/C and 936T/C polymorphism of vascular endothelial growth factor A(VEGF-A)gene. Methods 99 ROP infants(ROP group)and 80 premature infants(control group)were enrolled in this study. There was no difference of gestational age, birth weight and preoxygenation time between the ROP and control group (P＞0. 05). The peripheral blood was collected, polymorphism genotypes and frequency of VEGF-A+405 and VEGF-A936 were measured by pyrosequencing. Results There are CC, GG, CG genotypes in VEGF-A+405 site, while CC, CT genotypes in VEGF-A 936 site. The VEGF-A+405 gene allele of C, G were 92,106 with the frequencies of 46.5％, 53.5％ in the ROP group, and 90, 70 with the frequencies of 56.2％, 43.8％ in the control group; the difference between two groups was not statistically significant (X2 =3. 396, P=0. 066). There was no correlation between VEGF-A+ 405 polymorphism and ROP susceptibility (OR=0. 675, OR95％ CI=0. 444, 1. 026). The VEGF-A 936 gene allele of C, G were 32,166 with the frequencies of 16.2％, 83.8％ in the ROP group, and 16, 144 with the frequencies of 10.0％, 90.0％ in the control group; the difference between two groups was not statistically significant (X2=2.894, P=0.089). There was no correlation between VEGF-A 936 polymorphism and ROP susceptibility (OR=0. 768, OR95％ CI=0. 711, 0. 829). ConclusionThere is no correlation between VEGF-A+405 or VEGF-A 936 polymorphism and ROP susceptibility.