视网膜母细胞瘤患者外周血树突状细胞膜表面分子表达及其与免疫功能的相关性

目的 观察视网膜母细胞瘤(RB)患者外周血中树突状细胞(DC)细胞膜表面分子的表达及其与免疫功能的相关性。方法 分别采集50例正常健康儿童（对照组）及18例RB患者（RB组）外周血，培养、诱导扩增DC。采用同种混合淋巴细胞反应(MLR)检测对照组与RB组DC抗原呈递能力。采用流式细胞仪(FCM)检测两组DC细胞膜表面分子人类白细胞DR抗原(HLA-DR)、CD54及CD80的表达水平。制备25％（A组）、50％（B组）和75％(C组)的RB细胞上清液，培养对照组DC。培养后第10天，FCM检测不同浓度组DC细胞膜表面分子表达水平的变化情况。结果 MLR检测显示，随刺激细胞数目增多，DC抗原呈递能力逐渐增强。对照组DC抗原呈递能力较RB组明显增强，差异有统计学意义(P＜0.05)。对照组和RB组DC细胞膜表面分子HLA-DR、CD54及CD80的表达分别为12.14±2.52、34.89±5.12、10.93±3.1和7.33±2.20、25.28±4.54、7.89±3.75；RB组DC细胞膜表面分子表达较对照组低，差异均有统计学意义(t=4.07，3.96，2.59；P＜0.05)。A、B、C组DC细胞膜表面分子HLA-DR表达分别为9.95±2.55、6.48±1.82、3.11±1.47；CD54表达分别为34.75±4.92、21.25±3.44、15.41±3.52；CD80表达分别为9.15±2.18、5.05±2.01、2.90±1.10。与RB细胞培养上清液培养前比较，其细胞膜表面分子表达均有不同程度降低，并随着上清液浓度的增高，其下降程度更为明显(F=8.96，13.62，20.72;P＜0.05)。结论 RB患者外周血中DC细胞膜表面分子表达较正常健康者低，DC细胞功能缺陷与其细胞膜分子表达之间存在密切相关性。

The expression of molecules on surface of dendritic cells and its relationship with immune function in retinoblastoma patients

Objective To observe the expression of molecules on surface of dendritic cells (DC) in retinoblastoma (RB) patients, and investigate its relationship with immune function. MethodsThe peripheral blood of 50 normal subjects (control group) and 18 RB patients (RB group) were collected to proliferate the DC. The mixed lymphocyte reaction was performed on DC of the control and RB group to detect the antigen-presenting ability. The DC of control group was cultured in the supernate of SO-RB50with the different concentration of 25％ (group A), 50％ (group B) and 75％ (group C). Then the expression of HLA-DR, CD54 and CD80 on surface of DC were detected by flow cytometry (FCM). Results The results of MLR showed that DC antigen-presenting ability was gradually enhanced with the increase of stimulation of the cell. And the DC antigen-presenting ability of the control group was superior to that of the RB group (P＜0.05). The expression of HLA-DR, CD54 and CD80 on surface of DC in the control group (12. 14 ± 2. 52, 34. 89 ± 5. 12, 10. 93 ± 3. 1) were significantly higher than that in the RB group (7.33±2.20, 25. 28±4.54, 7. 89±3.75) (t=4.07, 3.96, 2.59; P＜0.05). The expression of HLA-DR,CD54 and CD80 on surface of DC in the group A, B and C (HLA-DR: 9.95±2. 55, 6. 48±1.82, 3.11±1.47; CD54: 34. 75±4.92, 21. 25±3.44, 15. 41±3.52; CD80: 9. 15±2.18, 5.05±2.01, 2. 90±1.10)were reduced in varying degrees compared with the control group; and with the increase of the concentration of supernate SO-RB50, the reduction was more evident (F=8.96, 13.62, 20. 72; P＜0.05). Conclusions The expression of molecules on surface of DC in RB patients is lower than that in the normal subjects. It is closely related to the functional deficiency of DC.