自体富血小板血浆促进兔上颌缝的牵张成骨

背景：富血小板血浆内含多种高浓度的生长因子，具有促进新骨生成，加速愈合的作用，对牵张成骨的作用尚无公识性结论。目的：观察自体富血小板血浆对免上颌骨牵张成骨的影响。设计、时间及地点：随机对照动物实验，于200707／08在中山大学北校区何母实验楼生理实验室完成。材料：3～5月龄健康家兔16只，雌雄各半，体质量1．4～1．7kg，随机分为实验组和对照组，每组8只。自制上切牙带环、外置式前牵引面具、牵引橡皮圈。凝结剂由1000U牛凝血酶溶于1mL100肌氯化钙制成。方法：分别在家兔左侧前颌缝的两侧置入钛钉（直径1．5ram），戴自制前牵引装置，试验组在牵张开始时，注射V（富血小板血浆）：V（凝结剂）=9：1混合的凝胶样物质至左侧前颌缝内。两组分别持续牵引1周和3周，每个时间点4只家兔。主要观察指标：牵引结束后测量骨缝两侧钛钉间距离的增加值以及组织学观察结果。结果：对照组和试验组家兔上颌均向前移位。同对照组相比，实验组骨缝标志钉间距离增加值较大，新骨生成与矿化较快，血管分布较多，牵张间隙中骨小粱较为粗壮和成熟。结论：富血小板血浆有助于骨组织再生，对兔上颌牵张成骨具有促进作用。

Autologous platelet-rich plasma promotes premaxillary suture distraction osteogenesis in rabbits

BACKGROUND: Platelet-rich plasma containing various high-concentration growth factors can promote new bone formation and accelerate bone healing. But its effects on distraction osteogenesis remain unclear. OBJECTIVE: To observe the effects of autologous platelet-dch plasma on premaxillary distraction osteogeaesis in rabbits. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at the Laboratory of Physiology, North Campus,Sun Yat-sen University between July and August 2007. MATERIALS: Sixteen healthy rabbits, the same number for male and female, aged 3-5 months,weighing 1. 4-1.7 kg,were randomly and evenly divided into an experimental group and a control group. Premaxillary incisor band,outside front distraction mask, and distraction rubber band were self-made.Coagulant was prepared by dissolving 1000 u bovine thrombin in 1 mL of 10% calcium chloride.METHODS: Titanium nails (1.5 mm in diameter) were separately inserted on two sides of left premaxillary suture in rabbits. A self-made distraction device was used. Gelatum-like substance [Vplatelet-rich plasma: V coagulant=9:1] was injected into the left premaxillary suture of experimental group rabbits immediately prior to distraction.In each group, one-week sustained distraction was performed in 4 rabbits,and three-week sustained distraction was performed in another rabbits. MAIN OUTCOME MEASURES: The increase of nail-nail distance on the two sides and histological results following distraction osteogenesis.RESULLTS: Rabbit premaxillary bone moved anterior in the two groups. The experimental group showed greater increase of nail-nail distance, faster bone formation and mineralization, more blood vessels, and thicker and more mature bone trabecula in the distraction interspace in comparison with the control group. CONCLUSION: Platelet-rich plasma will help bone tissue regeneration and promote premaxillary distraction osteogenesis.