牙龈卟啉单胞菌脂蛋白基因在慢性牙周炎及牙周健康者中的检测

目的 应用基因芯片技术检测3种脂蛋白基因PG0717、PG0183、PG2135在慢性牙周炎患者和牙周健康者牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)中的分布,探讨这些基因与牙周临床指数之间的关系,为研究脂蛋白在Pg致病过程中的作用提供依据.方法 选取41例慢性牙周炎患者(牙周炎组)及76例牙周健康者(健康对照组),记录探诊深度、附着丧失、探诊出血及牙齿松动度,取龈下菌斑进行细菌分离培养,以临床采集的样本提取的DNA为探针,以抑制消减杂交技术获得的PgW83特异基因片段PG0717、PG0183、PG2135为目标序列,采用Cy5荧光标记目标序列.应用基因芯片技术检测PG0717、PG0183、PG2135基因在牙周炎组病变部位、非病变部位和健康对照组Pg中的分布.结果在牙周炎组病变部位PG0717、PG0183、PG2135基因的检出率分别为90%(18/20)、70%(14/20)、70%(14/20),非病变部位的检出率分别为60%(12/20)、45%(9/20)、40%(8/20),而在健康对照组PG0717、PG0183、PG2135的检出率分别为55%(11/20)、25%(5/20)、30%(6/20).3种脂蛋白基因在牙周炎组病变部位和健康对照组中的检出率差异均有统计学意义(P＜0.05),并且与牙周探诊深度、临床附着丧失、探诊出血及牙齿松动度相关.结论 带有PG0717、PG0183、PG2135基因的Pg菌株致病力强.

Screening of lipoprotein genes of Porphyromonas gingivalis in chronic periodontitis patients

Objective To screen PG0717,PG0183 and PG2135 gene of Porphyromonas gingivalis(Pg)in subgingival plaque of the chronic periodontitis patients and periodontally healthy subjects and to investigate the relationship of these genes and periodontal clinical parameters.Methods Forty-one chronic periodontitis patients and 76 periodontally healthy individuals were included.Clinical parameters were measured and recorded by a single examiner,which included bleeding on probing(BOP),attachment loss(AL),probing depth(PD),and tooth mobility(TM).Subgingival plaque samples were taken by sterile subgingival curette.The three genes,which were present in PgW83 but absent from PgATCC33277 were labeled with Cy5 and used as probes in DNA microarray hybridization.The PG0717,PG0183 and PG2135 gene in affected region,normal region and healthy individuals of Pg was examined.Results The detecting rate of PG0717,PG0183 and PG2135 was 90%(18/20),70%(14/20)and 70%(14/20)respectively in affected region,60%(12/20),45%(19/20)and 40%(8/20)respectively in normal region and 55% (11/20),25%(5/20),30%(6/20)in Pg of healthy individuals.The detecting rate of PG0717,PG0183 and PG2135 was significantly different between the affected region and the healthy individuals(P＜0.05).Conclusions The Pg with genes of PG0717,PG0183,PG2135 was more pathogenic.