人脑缺血组织中IL-17表达及来源

目的证实IL-17是否参与人脑缺血损伤过程以及缺血脑组织中表达IL-17细胞的来源。方法选择疾病不同时间,尸检取因脑梗死死亡人的脑组织,以对侧正常脑组织为对照,通过免疫组织化学方法检测IL-17在人类脑组织中的表达。用线栓法封闭SD大鼠右侧大脑中动脉制作pMCAO动物模型。寡核苷酸原位杂交检测脑缺血不同时间点IL-17的表达;利用GFAP抗体和IL-17抗体双染方法检测大鼠脑组织中GFAP、IL-17共同表达细胞-神经胶质细胞。结果人脑缺血病灶中IL-17呈高表达,与对照侧脑组织相比存在极显著差异。IL-17表达高峰在发病后3~5d。SD大鼠缺血侧脑组织IL-17表达6h开始增高(P<0.01),第6天达到高峰。pMCAO手术侧可见大量IL-17和GFAP双染细胞。结论IL-17参与了人类及大鼠脑组织缺血性损伤的局部炎症反应过程;在大鼠实验中证实IL-17表达细胞来源于除以往确认的T淋巴细胞外,还包括神经胶质细胞。

The expression and source of IL-17 on human cerebral ischemic injury

Objective To confirm whether IL-17 plays a role in the process of human cerebral ischemic injury or not and to determine the source of IL-17 expressing cell during cerebral ischemic injury.Methods The levels of IL-17 in the ischemic hemisphere of human brain,which was removed at necropsy,were assayed immunohistochemically.In rats,permanent middle cerebral artery occlusion (pMCAO) was obtained by inserting nylon monofilament into the right external carotid artery and occluding the right middle cerebral artery.The expression of IL-17 mRNA in rat was assayed using oligoprobe in situ hybridization.IL-17 production by neuroglial cells was assayed by double-staining using antibody glial fibrillary acidic protein (GFAP)and antibody of IL-17.Results Levels of IL-17 were elevated in the ischemic hemispheres of human brain compared with the opposite normal hemispheres and peaked during 3d~5d after brain ischemia.The IL-17-positive cells were found in the ischemic lesion region.IL-17 mRNA was also elevated in ischemic hemispheres of pMCAO-operated rats,which were slightly elevated after 1h and peaked on 6d.IL-17 and GFAP double-stained were extensive in rat ischemic hemisphere. Conclusion The data suggested that IL-17 was mainly involved in the process of local inflammatory reaction of both human and rat ischemic brain injury,and also suggested that in additional to T cells,the neuroglial cell might be another cellular source of IL-17 in progression of cerebral ischemic injury in rats experiment.