Cross-reactive and major virus-specific epitopes are located at the N-terminal halves of the cylindrical inclusion proteins of turnip mosaic and zucchini yellow mosaic potyviruses |
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Authors: | A K Kundu K Ohshima N Sako H Yaegashi |
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Institution: | (1) Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga, Japan, JP;(2) Saga University, Saga, Japan, JP;(3) Laboratory of Plant Pathology, Faculty of Agriculture, Saga University, Saga, Japan, JP |
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Abstract: | Summary. To investigate the antigenic nature of cylindrical inclusion proteins (CIPs) of the potyviruses Turnip mosaic virus (TuMV) and Zucchini yellow mosaic virus (ZYMV), monoclonal antibodies (MAbs) against the two CIPs were produced and epitopes on the CIPs were localized using Escherichia coli-expressed CIP fragments in Western blot analysis. All 23 MAbs against ZYMV CIP reacted only with ZYMV CIP. In contrast out
of the 18 MAbs produced against TuMV CIP, 14 MAbs were TuMV CIP-specific while the remaining four MAbs cross-reacted with
both CIPs. The four cross-reactive MAbs recognized two distinct epitopes in the N-terminal half of TuMV CIP corresponding
to amino acid residues 103–119 and 224–237. Thirteen out of 14 TuMV CIP-specific MAbs recognized two distinct epitopes within
residues 1–102 and 120–214, while the other one recognized an epitope within residues 301–644. On the other hand, 21 out of
23 ZYMV CIP-specific MAbs recognized epitopes within residues 1–118, while the remaining two recognized epitopes within residues
301–522. These results suggest that cross-reactive and major virus-specific epitopes are located at the N-terminal half of
the respective CIPs.
Received August 10, 1999 Accepted March 2, 2000 |
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