| 人肺腺癌紫杉醇耐药细胞株交叉耐药性实验研究 |
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| 引用本文： | 钱军,杨爱珍,陈惠英,徐海军,秦叔逵. 人肺腺癌紫杉醇耐药细胞株交叉耐药性实验研究[J]. 医学研究杂志, 2008, 37(12): 52-54 |
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| 作者姓名： | 钱军 杨爱珍 陈惠英 徐海军 秦叔逵 |
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| 作者单位： | 解放军第81医院全军肿瘤中心,南京,210002 |
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| 摘 要： | 目的 通过建立体外肺腺癌耐药细胞株,研究紫杉醇交叉耐药性,为临床治疗提供有益的实验依据.方法 应用浓度递增和短时作用相结合,建立人肺腺癌紫杉醇耐药株SPC-A1/Taxol.通过MTT法检测加入不同药物后耐药细胞的半数抑制浓度,计算耐药指数.结果 SPC-A1/Taxol耐药细胞株对多西紫杉醇、长春瑞滨、长春新碱和阿霉素显示高度耐药,对喜树碱类和鬼臼毒素类显示中度或低度耐药,对铂类药物、抗代谢药和中药榄香烯乳未见交叉耐药性.结论 SPC-A1/Taxol耐药细胞株为一个较好的多药耐药体外模型,对其交叉耐药性的实验研究可以为临床用药提供有益依据.
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| 关 键 词： | 肺肿瘤 多药耐药性 紫杉醇 细胞株 |
Experimental Research of Cross Resistance About Paclitaxel Resistant Human Lung Adenoearcinama Cell. |
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| Qian Jun,Yang Aizhen,Chen Huiying,Xu Haijun,Qin Shukui. Experimental Research of Cross Resistance About Paclitaxel Resistant Human Lung Adenoearcinama Cell.[J]. Journal of Medical Research, 2008, 37(12): 52-54 |
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| Authors: | Qian Jun Yang Aizhen Chen Huiying Xu Haijun Qin Shukui |
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| Affiliation: | Qian Jun,Yang Aizhen,Chen Huiying,Xu Haijun,Qin Shukui.Oncolgy Center of PLA,the 81st Hospital,Nanjing 210002,China |
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| Abstract: | Objective To investigate paclitaxel cross resistance and provide information for clinical therapy by establishing a paclitaxel resistant human lung adenocarcinoma cell line.Method A paclitaxel resistant human lung adenocarcinoma cell line named with SPC-A1/Taxol was developed by intermittent exposure to gradually increasing concentration of taxol.Resistant index was calculated from median inhibitory concentration(IC50)which was evaluated by MTT assay.Results SPC-A1/Taxol cell line displayed high resistance ... |
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| Keywords: | Lung tumor Multidrug resistance Paclitaxel Cell line |
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