hMAM RNA 和CEA RNA T-PCR检测乳腺癌外周血微转移

目的应用RT-PCR法检测乳腺癌患者外周血中的human ammaglobin(MAM)mRNA和CEA RNA表达情况.方法用RT-PCR法,检测52例乳腺癌患者外周血中hMAM RNA和CEA RNA的表达情况,另抽取28例乳腺良性疾病患者和10例健康志愿者外周血标本作为对照.结果 52例乳腺癌患者外周血中hMAM RNA和CEA RNA的阳性率分别为30.8％、34.6％.28例乳腺良性疾病患者和10例健康志愿者外周血中hMAM RNA、CEA RNA表达均为阴性.两者的阳性表达均与患者的腋淋巴结状况、TNM分期差异有显著性意义(P<0.05).而与患者的年龄、原发肿瘤大小、病理类型以及激素受体状况等均无显著性差异(P＞0.05).结论 hMAM RNA、CEA RNA均可作为标志物来检测乳腺患者外周血中微转移,联合检测可增加外周血中循环肿瘤细胞的检测率.

Detection of breast cancer micrometastases in peripheral blood by using hMAM mRNA and CEA mRNA reverse transcription-polymerase chain reaction

Objective Detection of breast cancer micrometastasis in peripheral blood by using human mammaglobin(hMAM) mRNA and CEA mRNA RT PCR. Methods Expressions of hMAM mRNA and CEA mRNA in peripheral blood were detected in 52 cases of breast cancer, and 28 patients with breast benign lesions and 10 heathly volunteers as controls by RT PCR. Results The positive hMAM mRNA and CEA mRNA expession were 30.8% and 34.6%, No positive hMAM mRNA and CEA mRNA were found in peripheral blood of 28 patients with breast benign lesions and 10 heathly volunteers. The hMAM mRNA and CEA mRNA expessions were correlated with axillary lymph nodes status and TNM stage. There was no significant difference between micrometastasis and any of other clinical parameters. Conclusion The hMAM mRNA and CEA mRNA may be potential tumor markers for detecting micrometastasis and recurrence in peripheral blood.