| 直接注射含戊型肝炎病毒cDNA质粒引发小鼠抗体反应 |
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| 引用本文： | 鲁凤民,庄辉.直接注射含戊型肝炎病毒cDNA质粒引发小鼠抗体反应[J].中国公共卫生学报,1995,14(1):41-42. |
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| 作者姓名： | 鲁凤民 庄辉 |
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| 作者单位： | 北京医科大学流行病学教研室 |
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| 摘 要： | 将由ＳＶ４０病毒晚期启幼子调控的戊型肝炎病毒第三开放读码框架（ＯＲＦ３）ｃＤＮＡ质粒ｐＳＶＬ－ＨＥＶＯＲＦ３直接注射到小鼠骨骼肌肉，引发了小鼠抗－ＨＥＶ抗体反应，为ＨＥＶ基因疫苗的研制提供了可能性。
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| 关 键 词： | 戊型肝炎病毒，基因疫苗，免疫应答 |
Elicitation of an Antibody Response by Direction with a Plasmid Containing HEV cDNA |
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| Authors: | Lu Fengmin |
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| Institution: | Lu Fengmin,et al., |
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| Abstract: | Gene vaccine,or antigen-expression DNA for elicitation of a specific immune response, represents s new approach to immunization. In this study, a plasmid containing hepatitis E virus (HEV) genome ORF3, driven by SV40 late promoter,has been tested for the ability to elicite the antibody response against HEV in mice. After 1-2 direct injections of 100 ug plasmid DNA into skeletal muscles of mice hind legs, all 3 mice inoculated were positive for anti-HEV IgG in sera by enzyme-linked immunoassay (ELISA). The highest anti-HEV titer reached 1: 20 dilution. This study indicates that the direct inoculation of the plasmid DNA containing HEV cDNA into mice muscles is able to elicite a specific antibody response aganist HEV. |
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| Keywords: | HEV Gene vaccine Immune respose |
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