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Use of panel testing for detection of antinuclear antibody in a resource-limited setting: an appraisal |
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| Authors: | Ranjana Walker Minz Biman Saikia Shashi Anand Subhash Varma Surjit Singh |
| Affiliation: | 1. Department of Immunopathology, Post Graduate Institute of Medical Education &2. Research, Chandigarh, India;3. Department of Internal Medicine, Post Graduate Institute of Medical Education &4. Department of Rheumatology, Post Graduate Institute of Medical Education & |
| Abstract: | Objectives: Despite an increase in the incidence of systemic connective tissue diseases (CTD), panel testing for detection of antinuclear antibodies (ANA) is not a routine practice in many health centers of the Indian subcontinent. Consequently, the data on its significance is scanty. Methods: To evaluate utility of panel testing, line immunoassay (LIA) and indirect immunofluorescence antinuclear antibody test (IIF-ANA) were performed in 321 cases of CTD. Results: Out of 321 serum samples screened by the above tests, 227 were positive and 18 were negative by both LIA and IIF-ANA. Additional 11/321 (3.4%) cases were picked up by LIA. SSA was most common specificity in these cases followed by SSA/SSB, SSB, Ro-52, Jo-1, dsDNA and nRNP/Sm. Conclusion: Use of LIA along with IF-ANA and ELISA improves sensitivity of CTD screening. |
| Keywords: | CTD ANA LIA Indirect immunofluorescence dsDNA ELISA |