Transformation of Fulvia fulva,a fungal pathogen of tomato,to hygromycin B resistance |
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| Authors: | R P Oliver I N Roberts R Harling L Kenyon P J Punt M A Dingemanse C A M J J van den Hondel |
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| Institution: | (1) Norwich Molecular Plant Pathology Group, School of Biological Sciences, University of East Anglia, NR4 7TJ Norwich, GB;(2) Medical Biology Laboratory TNO, P.O. Box 45, NL-2280 AA Rijswijk, The Netherlands |
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| Abstract: | Summary A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integrated into the genome. The number and sites of integrations varied among transformants. The demonstration of transformation opens the way for the molecular genetic analysis of the interaction of Fulvia with tomato. |
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| Keywords: | Tomato leaf mould Cladosporium fulvum Deuteromycete |
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