8-羟基鸟嘌呤糖苷酶1基因多态性与喉癌易感性的关系

目的:探讨8-羟基鸟嘌呤糖苷酶1基因(hOGG1)Ser326Cys多态性与喉癌遗传易感性的关系.方法:采用病例-对照设计,选择72例喉鳞状细胞癌患者(病例组),并与72例性别比一致,平均年龄相差＜5岁的对照组进行比较.采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析hOGG1c.326多态性.结果:病例组hOGG1c.326杂合型及突变(Ser/Cys Cys/Cys)比例高于对照组(P＜0.05),携带hOGG1c.326Ser/Cys Cys/Cys基因型个体较携带hOGG1c.326Ser/Ser基因型的个体患喉癌的风险升高了2.54倍.结论:hOGG1c.326(Ser/Cys或Cys/Cys)基因型可能是喉癌发病的风险因子,其可作为一种易感性分子标志物,对喉癌的早期预防具有一定的参考价值.

Association of Human 8-Oxoguanine Glycosylase 1 Polymorphism with the Susceptibility of Laryngeal Cancer

Objective: To explore the association between hOGG1 gene polymorphisms and the risk for laryn-geal carcinoma. Methods: A case-control study was conducted. Seventy-two laryngeal squamous carcinoma and 72 healthy controls who were matched by sex and age between two groups were included in study. Polymerase chain reaction and Restric-tion Fragment Length Polymorphism (PCR-RFLP) were used to analyze the hOGG1c.326Ser326Cys polymorphisms. Results: The frequency of hOGG1c.326Ser/Cys Cys/Cys genotypes in the case group was higher than that in control group(P < 0.05). There was a 2.54-fold increased risk of laryngeal carcinoma for individuals carrying hOGG1c.326Ser/Cys Cys/Cys genotypes, compared with subjects carrying hOGG1c.326Ser/Ser genotype. Conclusion: The polymorphism of hOGG1Ser326Ser/Cys Cys/Cys might be associated with the susceptibility of laryngeal cancer. As a molecular biomarker, hOGG1 gene c.326Ser/Cys Cys/Cys genotypes may be useful for the susceptibility detecting of laryngeal cancer.