RAG-1在小鼠胚胎脑发育中的表达

目的:观察小鼠脑组织正常发育期间重组激活基因1(RAG-1)表达的时序变化,并对其进行组织定位。方法:分别取孕11d、13d、15d、17d、19d,新生(P0)和成年的小鼠脑组织,提取总RNA,用RT-PCR观察RAG-1表达的时序变化;同时,经冠状切面制作各组脑组织的连续冰冻切片,进行RAG-1免疫组织化学染色。结果:RAG-1自小鼠胚胎11d开始出现少量表达,以后表达逐渐增加,19d到达高峰,RT-PCR显示结果与免疫组化结果基本一致。RAG-1蛋白主要表达在发育过程中的杏仁核、下丘脑、丘脑、海马等区域,大脑皮质的表达逐渐出现于室管膜层、中间层、室管膜下层和皮质板层。结论:在小鼠胚胎脑发育期间,RAG-1表达时序和脑发育一致,而且表达高于成年鼠。

Expression of RAG-1 in brain during mouse development

Objective: To investigate the expression of recombination activating gene-1 (RAG-1) and its localization in the mouse brain during the embryonic development. Methods: The brain tissues of E(embryonic day) 11,E13,E15,E17,E19,P0(the birth day) and adult mice were taken,the total RNA of brains were extracted and the changes of RAG-1 expression were detected with the method of RT-PCR.The freeze sections of brain tissues from each group were stained with immunohistochemistry method. Results: The expression of RAG-1 persisted from E11 to P0 brain and was steadily increased from E11 to E19; the results of RT-PCR were similar to that of immunohistochemistry.The positive-cells mainly appeared in the nucleus amygdalae,hypothalamus,thalamus and hippocampus at developmental stage.The expression began to appear in ventricular zone(VZ) and intermediate zone(IZ) of telecephalic vesicle,then gradually increased in subventricular zone(SVZ),corticle plate(CP) and subcorticle plate (SP). Conclusion:The expression of RAG-1 in mouse embryonic brain tissue is higher than that in the adult mouse,which may be related to the process of neuron development.