| 骨髓内皮细胞无血清条件培养液对CFU—E和BFU—E生成的影响 |
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| 引用本文： | 黄艳红 李卫民. 骨髓内皮细胞无血清条件培养液对CFU—E和BFU—E生成的影响[J]. 湖南医科大学学报, 2000, 25(2): 201-202 |
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| 作者姓名： | 黄艳红 李卫民 |
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| 摘 要： | 利用本室建立的小鼠骨髓内皮细胞株作传代培养，收集其无血清条件培养液，离心超滤，观察分子量（ＭＷ）〉１０００００组分及分子量〈１０００００组分对红系生成的影响。结果表明：分子量〉１０００００组分对ＣＦＵ－Ｅ和ＢＦＵ－Ｅ有明显刺激作用，且在２％～６％（Ｖ／Ｖ，下同）范围内集落产率随浓度增加而增加，而分子量〈１０００００组分对ＣＦＵ－Ｅ，ＢＦＵ－Ｅ有明显抑制作用，且在１０％～４０％范围内集落产率随浓度增
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| 关 键 词： | 骨髓细胞 内皮细胞 造血细胞 CFU－E BFU－E |
Effects of serum-free murine bone marrow endothelial cell conditioned medium on the growth of CFU-E and BFU-E] |
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| Y H Huang,W M Li,D Z Jiang,Q R Wang. Effects of serum-free murine bone marrow endothelial cell conditioned medium on the growth of CFU-E and BFU-E][J]. Bulletin of Hunan Medical University, 2000, 25(2): 201-202 |
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| Authors: | Y H Huang W M Li D Z Jiang Q R Wang |
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| Affiliation: | Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078. |
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| Abstract: | Serum-free murine bone marrow endothelial cell conditioned medium(mBMEC-cm) was collected. The mBMEC-cm was ultrafiltered in Centriprep. The concentrated retentive substance of mBMEC-cm (MW > 10,000) and filtrate of mBMEC-cm(MW < 10,000) were obtained. The effects of mBMEC-cm on the growth of CFU-E and BFU-E were investigated. The results showed: 1. The retentive substance of mBMEC-cm significantly enhanced the growth of CFU-E and BFU-E. The effects of mBMEC-cm(2%-6%, V/V) on the growth of CFU-E and BFU-E were dose-dependent. 2. The filtrate of mBMEC-cm markedly inhibited the growth of CFU-E and BFU-E. The effects of the filtrate of mBMEC-cm on the growth of CFU-E and BFU-E were also dose-dependent. The mechanism of regulation will be studied further. |
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