电针足三里穴对正常大鼠5- HT的影响

目的:通过观察电针足三里穴对正常大鼠血清、下丘脑中5 - HT含量以及胃、肠、脑组织5-HT表达的影响,研究足三里穴与胃特异性机制.方法:按随机原则将40只SD大鼠分为正常对照组,三阴交1、2组,足三里1、2组,共5组,每组8只.高效液相法检测血液及下丘脑中5 - HT含量,免疫组化法检测5 - HT在胃、肠、脑组织中的表达.结果:足三里1、2组5- HT含量明显高于正常对照组(P＜0.05),且足三里1、2组分别高于三阴交1、2组(P＜0.05).胃、肠、脑组织中5- HT阳性表达IOD,足三里1、2组高于正常对照组(P＜0.05),足三里组高于三阴交组(P＜0.05).结论:电针足三里穴能有效调节正常大鼠血清及组织中5 - HT的变化,具有穴位特异性,提示足三里穴与胃之间存在相关性,为穴位脏腑相关理论提供科学依据.

Effect of Electroacupuncture at Zusanli on 5-HT of Normal Rats

Objective:To study the special interation between Zusanli and stomach by observing the effect of electroacupuncture(EA) at Zusanli on 5-HT of normal rats.Methods:40 SD rats were randomly divided into normal control group,Sanyinjiao 1 and 2 group,Zusanli 1 and 2 group,with 8 cases in each group.The content of 5-HT in the blood and tissue was detected with the methods of high performance liquid chromatography.Methods of immunohistochemistry and in situ hybridization were used to detect the expression of 5-HT.Results:The content of 5-HT in Zusanli 1,2 group were significantly higher than that in the control group(P<0.05),and higher in Zusanli than that in Sanyinjiao group respectively(P<0.05).The expression of 5-HT of the stomach,intestine,brain in Zusanli 1,2 group was higher than that in the control group(P<0.05),and higher in Zusanli than that in Sanyinjiao group(P<0.05).Conclusion:The content of 5-HT of normal rats can be regulated by EA at Zusanli.It can be verified that Zusanli is a specific point to stomach,the special relations exist in Zusanli and stomach.That can provide scientific evidence for the theory of acupoint and visceral coupling.