Effect of polychlorinated biphenyls (PCBs) administration on phospholipid biosynthesis in rat liver |
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Authors: | Kozo Ishidate Yasuo Nakazawa |
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Institution: | Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan |
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Abstract: | The effect of PCBs or phenobarbital on the biosynthesis of phospholipids in hepatic endoplasmic reticulum of rats was studied by the intraperitoneal injection of 32P]orthophosphate, Me?14 C]choline or 2?3H]glycerol. Significant increases in liver microsomal phospholipid content after the administration of either PCBs or phenobarbital indicated the actual proliferation of endoplasmic reticulum membranes. The rate of both 32P] and 14C] incorporations into microsomal choline-containing phospholipids, such as phosphatidylcholine, sphingomyelin and lysophosphatidylcholine, was reduced to one fifth by PCBs administration compared with control animals. The incorporation of 32P]orthophosphate into phosphatidylethanolamine or other phospholipid classes was less or not affected, respectively, by PCBs administration. The specific inhibitory effect of PCBs on the incorporation into cholinecontaining phospholipids was not observed when 2?3-H]glycerol was used as a precursor. Phenobarbital administration, however, increased significantly the rate of 32P] incorporation into liver phospholipids, especially phosphatidylcholine. It is suggested that the increase in microsomal phospholipid content by PCBs administration is not due to the stimulation of synthesis but to the inhibition of the catabolism of membrane phospholipids and that the increase in content caused by phenobarbital is due at least in part, to the stimulation of synthesis. The possible site(s) of PCBs-induced inhibition of phospholipid biosynthesis in rat liver is discussed. |
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Keywords: | PL phospholipid(s) PC phosphatidylcholine PE phosphatidylethanolamine PI phosphatidylinositol PS phosphatidylserine Sph sphingomyelin DPG diphosphatidylglycerol (cardiolipin) LPC lisophosphatidylcholine NL neutral lipids PB phenobarbital |
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