Dihydroavenanthramide D prevents UV‐irradiated generation of reactive oxygen species and expression of matrix metalloproteinase‐1 and ‐3 in human dermal fibroblasts |
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| Authors: | Kang‐Beom Kwon Bo‐Mi Hwang Jin‐Ki Hwang Yong‐Ouk You Min Seuk Kim Wan Lee Jeong‐Ho Lee Hye‐Jung Kim Jong‐Suk Kim Young‐Rae Lee |
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| Affiliation: | 1. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, , Iksan, South Korea;2. Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, , Jeonju, South Korea;3. Department of Oral Biochemistry, School of Dentistry, Wonkwang University and Institute of Wonkwang Dental Research, , Iksan, South Korea;4. Laboratory of Oral Physiology, School of Dentistry, Wonkwang University, , Iksan, South Korea;5. Department of Oral & Macillofacial Radiology, School of Dentistry, Wonkwang University, , Iksan, South Korea;6. Department of Herbal Medicine Resources, College of Environmental and Bioresource Sciences, Chonbuk National University, , Iksan, Korea;7. Department of Family Medicine, The Catholic University of Korea, Incheon St. Mary's Hospital, , Incheon, South Korea |
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| Abstract: | Ultraviolet B (UVB) radiation induces photoageing by upregulating the expression of matrix metalloproteinases (MMPs) in human skin cells. Dihydroavenanthramide D (DHAvD) is a synthetic analog to naturally occurring avenanthramide, which is the active component in oats. Although anti‐inflammatory, anti‐atherosclerotic and antioxidant effects have been reported, the antiphotoageing effects of DHAvD are yet to be understood. In this study, we investigated the inhibitory effects of DHAvD on UVB‐induced production of reactive oxygen species (ROS) and expression of MMPs, and its molecular mechanism in UVB‐irradiated human dermal fibroblasts. Western blot and real‐time PCR analyses revealed that DHAvD inhibited UVB‐induced MMP‐1 and MMP‐3 expression. It also significantly blocked UVB‐induced ROS generation in fibroblasts. Additionally, DHAvD attenuated UVB‐induced phosphorylation of MAPKs, activation of NF‐κB and AP‐1. DHAvD regulates UVB‐irradiated MMP expression by inhibiting ROS‐mediated MAPK/NF‐κB and AP‐1 activation. DHAvD may be a useful candidate for preventing UV light‐induced skin photoageing. |
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| Keywords: | DHAvD
MAPK
MMP
ROS
UVB
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