HCV抗体检测临界值附近样本传播HCV风险的研究

目的用尽可能低的消耗，达到HCV感染早期诊断，尽量缩短ELISA HCV抗体检测的“窗口期”，防止和减少经输血传播丙型肝炎的风险。方法采集初次检测抗-HCV（S／CO）样本测定值／临界值0．40～0．99的样本310份，采用“HCV抗体分片段酶联免疫确认试剂盒”进行补充旁证检测，对检测阳性及可疑阳性样本再进行HCV—PCR和RIBA及HCV—cAg检测。结果310份样本共检出单片段阳性样本25份，2个片段以上阳性样本3份，共计28份。HCV—PCR阳性3份，HCV—cAg阳性4份，RIBA检测3个条带阳性2份，1个条带阳性22份。28份样本中，抗-HCV分片段、PCR、HCV—cAg以及RIBA共同阳性1份，HCV—cAg与PCR共同阳性2份，HCV—cAg和RIBA共同1份。结论加强对抗-HCV检测阴性高值样本的重视，尽量缩短ELISA—HCV抗体检测“窗口期”。

Research on the Spread Risk of HCV Antibody Samples Near the Cut off Value

Objective To diagnose early infection of HCV with expense as low as possible and to shorten the ＂preseroconversion window phase （PWP）＂ of anti-HCV detection in present condition for decrease risk through blood spread. Methods 310 samples which anti-HCV ELISA S/CO values were 0. 4-0.99 were collected. The plasma of the 310 samples were separated and stored at -30℃. The plasma was tested by anti-HCV different fragment ELISA assay kit additionally. The positive and probable positive samples were tested further using HCV-PCR, RIBA and HCV-cAg respectively. Results Among 310 serum samples,25 serum samples had only one fragment positive, and 3 serum samples had more than two fragments positive. Among them, 3 samples were HCV-PCR positive, 4 samples were HCV-cAg positive,2 sam- ples had three lines positive by RIBA and 22 samples had only one line by RIBA. One sample of the above-mentioned 28 serum samples was detected positive by anti-HCV different fragment assay kit,HCV-PCR,HCV-cAg and RIBA simultane- ously. Two samples were positive in HCV-cAg and HCV-PCR assays. One sample was positive in HCV-cAg and RIBA assays. Conclusion In present condition, in order to detect HCV infection more earlier than ELISA-HCV antibody detection and to shorten the PWP, the anti-HCV critical value negative samples should be detected by anti-HCV different fragment assay kit and HCV-PCP, simultaneously. The combination detection of anti-HCV different fragment assay kit and HCV- PCR assay could take the place of the expensive import RIBA assay kit.