Biocompatibility of KLD-12 peptide hydrogel as a scaffold in tissue engineering of intervertebral discs in rabbits |
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| Authors: | Jianhua Sun Qixin Zheng Yongchao Wu Yudong Liu Xiaodong Guo Weigang Wu |
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| Affiliation: | 1. Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Department of Orthopedics, the First Affiliated Hospital of Shihezi University, Xinjiang 832008, China
2. Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | KLD-12 peptide with a sequence of AcN-KLDLKLDLKLDL-CNH2 was synthesized and its biocompatibility was assessed in animals.Rabbit MSCs were cultured in the hydrogel for 2 weeks.Live cells were counted by using Calcein-AM/PI fluorescence staining.MTT was employed to assess the viability of MSCs cultured in KLD-12 peptide solution of 0.01%,0.03%,and 0.05%.Hemolysis test,skin irritation test and implantation test were conducted to evaluate its biocompatibility with host tissues.Our results demonstrated that the ... |
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| Keywords: | peptide self-assembly biocompatible materials intervertebral disc |
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