乙型肝炎病毒感染者血清干扰素诱导基因20 kDa蛋白表达水平及其临床意义

目的观察乙型肝炎病毒感染者血清干扰素诱导基因20 kDa蛋白（ISG20）蛋白表达变化及其临床意义。 方法选取2017年3月至2018年6月汉中市中心医院收治的HBV感染所致慢性乙型肝炎患者（CHB组）67例、肝硬化患者（LC组）58例、肝癌患者（HCC组）54例和同期体检47例健康者（对照组）。采用酶联免疫吸附法检测各组研究对象血清ISG20蛋白水平。比较不同组患者血清ISG20水平差异。采用Spearman相关分析探讨血清ISG20水平与各组患者年龄、性别、红细胞计数、白细胞计数、血小板计数、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、总胆红素（TBil）、直接胆红素（DBil）、白蛋白、凝血酶原、甲胎蛋白（AFP）、HBV DNA的相关性。 结果对照组患者血清ISG20水平为9.4（0.82～25.72）ng/ml,显著低于CHB组[18.6（1.87～56.32）ng/ml,Z =－1.567、P = 0.034]和HCC组[28.2（3.09～81.42）ng/ml,Z = －1.854、P = 0.021]。HCC组患者血清ISG20水平显著高于CHB组（Z =－1.431、P = 0.041）和LC组[12.9（2.81～77.54）ng/ml,Z =－1.987、P = 0.029）。HCC组不同Child-Pugh分级患者血清ISG20水平差异有统计学意义（H = 6.976、P = 0.031）。HCC组患者血清ISG20水平与AST（r = 0.323、P < 0.001）、ALT（r = 0.248、P = 0.036）、TBil（r = 0.221、P = 0.031）、DBil（r = 0.215、P = 0.043）、AFP（r = 0.176、P = 0.044）呈正相关,与白蛋白呈负相关（r =－0.239、P = 0.019）。 结论HBV感染者,尤其是HBV感染所致HCC患者,血清ISG20水平升高。血清ISG20水平与HBV感染疾病进展和临床参数有一定的相关性。

Clinical significance and level of serum interferon-stimulated gene 20 kDa protein in patients with hepatitis B virus infection

ObjectiveTo investigate the expression and clinical significance of serum interferon-stimulated gene 20kDa protein (ISG20) of patients with hepatitis B virus infection. MethodsTotal of 67 patients with chronic hepatitis B (CHB group), 58 patients with liver cirrhosis (LC group), 54 patients with liver cancer (HCC group) and 47 healthy controls were selected in Hanzhong Central Hospital from March 2017 to June 2018. Serum ISG20 protein levels were detected by enzyme-linked immunosorbent assay. The difference of serum ISG levels in different groups were compared. The serum ISG20 level and age, gender, count of red blood cell, count of white blood cell, platelet count and aspartate aminotransferase, alanine aminotransferase, total bilirubin (TBil), direct bilirubin (DBil), albumin, prothrombin, alpha-fetoprotein (AFP) and HBV DNA load of different groups were analyzed by Spearman’s correlation analysis. ResultsThe serum ISG20 level in control group was 9.4 (0.82-25.72) ng/ml, which was significantly lower than that of the CHB group [18.6 (1.87-56.32) ng/ml; Z =-1.567, P = 0.034] and the HCC group [28.2 (3.09-81.42) ng/ml, Z =-1.854, P = 0.021]. The serum ISG-20 level in HCC group was significantly higher than that of CHB group (Z =-1.431, P = 0.041) and LC group [12.9 (2.81-77.54 ) ng/ml; Z =-1.987, P = 0.029). There was significant difference of serum ISG20 level between patients with different Child-Pugh grades in HCC group (H = 6.976, P = 0.031). Serum ISG20 levels of patients in HCC group were positively correlated with AST (r = 0.323, P < 0.001), ALT (r = 0.248, P = 0.036), TBil (r = 0.221, P = 0.031), DBil (r = 0.215, P = 0.043) and AFP (r = 0.176, P = 0.044); but negatively correlated with albumin (r =-0.239, P = 0.019). ConclusionsSerum ISG20 levels were elevated in patients with HBV infection, especially in patients with HCC caused by HBV infection. Serum ISG20 levels had a certain correlation with progression and clinical parameters of HBV infection diseases.